Harpins Protein Alleviate Phytophthora megasperma by Adjusting PAL Enzyme Activity in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2011年03期

Page:

526-528

Research Field:

Publishing date:

Info

Title:

Harpins Protein Alleviate Phytophthora megasperma by Adjusting PAL Enzyme Activity in Soybean

Author(s):

SHEN Hong-bo¹; HU Zhi-feng¹; DING Jun-jie²; YU Yong-mei¹; GU Xin²; YANG Xiao-he²

1. Heilongjiang Agricultural Vocational and Technical College, Jiamusi 154007;

2.Ministry of Agriculture, Harmful Biology of Crop Scientific Monitoring Station Jiamusi Experiment Station/Jiamusi Branch, Heilongjiang Academy of Agricultural Sciences, Jiamusi 154007,Heilongjiang, China

Keywords:

Soybean;  Phytophthora megasperma; Harpins protein;  phenylalnine ammonialyase (PAL)

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2011.03.0526

Abstract:

The soybean resistance to Phytophthora megasperma could be significantly improved by spraying Harpins protein. In order to make clear the resistance mechanism of Harpins protein to Phytophthora megasperma, we sprayed Harpins protein on soybean seedling of different resistance soybean varieties, and measured the dynamic changes of phenylalnine ammonialyase (PAL) in soybean root after spraying within 120 hours later. The results showed that Harpins protein increased PAL activities of tested varieties at 72 h after spraying, with those in susceptible ones significantly higher than resistant ones. For susceptible varieties, PAL activity showed increasing-and-then-decreasing trend and peaked at 72 h after spraying Harpins protein; while the PAL activity showed slowly increasing trend in susceptible varieties.

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