A Fast Detection Method for Screening of Lipoxygenase Null Individual Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2011年03期

Page:

484-487

Research Field:

Publishing date:

Info

Title:

A Fast Detection Method for Screening of Lipoxygenase Null Individual Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis in Soybean

Author(s):

WANG Shao-dong¹;  JIANG Yan¹;  WANG Hao¹;  LIU Wei¹;  LI Yuan-ming¹;  LI Hong-wei¹;  LI Wen-bin²

1. National Research Center of Soybean Engineering and Technology (NRCSET), Northeast Agricultural University, Harbin 150030;

2. Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin 150030, Heilongjiang, China

Keywords:

Lipoxygenase null; Sodium dodecyl sulfate polyacrylamide gel electrophoresis; Screening of filial generation; Soybean

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2011.03.0484

Abstract:

In this research, through the improvement of the sample pretreatment and confection of the gel, a fast detection method were developed for screening of lipoxygenase(Lox) null individual using sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) in soybean. By this method, we have screened 27 strains of the Lox-1,-2,-3 null, and some Lox-1,-3 or Lox-2,-3 incompletion lacking individuals from 289 individuals in the F₂(CS8000（♀）×FJ307(♂))generations. The results proved that the method was better in precision, velocity and less costs of the screening. It is significant for selecting the lipoxygenase null individual in quality improvement breeding of soybean.

References:

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