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Molecular Detection and Aphid Resistance Analysis of Later Generation of Transgenetic Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年03期
Page:
455-458
Research Field:
Publishing date:

Info

Title:
Molecular Detection and Aphid Resistance Analysis of Later Generation of Transgenetic Soybean
Author(s):
LIU Xian-wen;  WANG Biao;  YAO Lu-ming;  XIAO Liang;  WU Tian-long
Plant Science Department, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240， China
Keywords:
Transgenic soybean;  Molecular identification;  Resistance to aphids
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.03.0455
Abstract:
The target genes in high generations of transgenic soybean were detected by PCR amplification and their resistances to aphid were also investigated. The results showed that in co-transformation of three genes, there were 409 plants with single gene, 179 plants owning double genes and 36 plants having three genes, accounted for 37.8%, 17.0% and 3.4%of total detecting plants, respectively; the number of plants only with pta gene were the most, 239 plants accounted for 22.6%of total plants. The number of aphids was up to the highest after inoculated on transgenic soybean for 15 days, the range of 37 to 66 in eight transgenic lines, but the control had 154 aphids. Segregation in high generations of transgenic soybean was obvious using gene co-transformation, but resistance to aphids in their homozygous progenies was still significant.

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