Biochemical Mechanism of Resistance against Soybean Cyst Nematode Induced by Plant Growth Promoting Rhizobacteria in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2011年03期

Page:

442-446

Research Field:

Publishing date:

Info

Title:

Biochemical Mechanism of Resistance against Soybean Cyst Nematode Induced by Plant Growth Promoting Rhizobacteria in Soybean

Author(s):

DUAN Yu-xi¹; ZHANG Yu¹; ZHU Xiao-feng¹; LIU Da-wei¹; LI Song¹; CHEN Li-jie¹; WANG Yuan-yuan²

1.Nematology Institute of Northern China， Department of Plant Protection，Shenyang Agricultural University;

2.Department of Biological Science and Technology，Shenyang Agricultural University，Shenyang 110866, Liaoning, China

Keywords:

PGPR;  Induced resistance; Heterodera glycines; Defense enzymes; Chitinase; Total phenolics

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2011.03.0442

Abstract:

It has been a new research focus on biological control that the induction of disease resistance and growth response in plants is elicited by plant growth promoting rhizobacteria（PGPR）. This study aimed to examine the biochemical mechanism of the resistance to soybean cyst nematode(Heterodera glycines Ichinohe) in soybean induced by PGPR [Sneb207(Bacillus megaterium) and Sneb482(Bacillus megaterium)].Seed bacterization with Sneb207 and Sneb482 were utilized in the experiment. The soybean plants were inoculated with eggs of soybean cyst nematode after soybean trefoil stage and the samples of roots were obtained 6,12,18,24 and 30 days later. Activities of plant resistance correlated enzymes including defense enzymes, namely phenylalanine ammonia-lyase (PAL), polyphenoloxidase (PPO), peroxidase (POD) and chitinase were measured, the content of total phenolics was also determined. The results showed that both the activities of PAL, PPO, POD, chitinase and the contents of total phenolics could be increased significantly by seed coating with fermentation liquid of plant growth promoting rhizobacteria (Sneb207, Sneb482) in the soybean roots than those in control. Sneb207 showed greater potential in induced resistance against soybean cyst nematode in the soybean plants than Sneb482.

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