|Table of Contents|

Susceptibility of Soybean Cotyledonary Node and Seedlings to NaCl Stress(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年03期
Page:
438-441
Research Field:
Publishing date:

Info

Title:
Susceptibility of Soybean Cotyledonary Node and Seedlings to NaCl Stress
Author(s):
ZHANG Yun-yue;  WANG Peng;  WANG Pi-wu;  FU Yong-ping;  LI Bo
Biotechnology Center of Jilin Agricultural University, Changchun 130118, Jilin, China
Keywords:
Soybean;  Genotype;  NaCl;  Screening
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.03.0438
Abstract:
During plant genetic transformation, the use of marker gene is an efficient method to select and identity transgenic plants. Using safe selectable marker not only screened transformants but also decreased potential risk of genetically modified food. In this study, NaCl was regarded as selectable marker. The effects of NaCl on cotyledonary node bud differentiation, rooting of shoots and growing of soybean seedling were investigated using five soybean varieties. The results showed that the effects of NaCl on different genotypes and explants exist differences. For cotyledonary node,the critical concentration of NaCl was 225 mmo·L－1 for Jinong 27 and Jinong 17; 200 mmo·L－1 for Jilin 30 and DE2259; 175 mmo·L－1 for Jinong 28. For rooting, the critical concentration of NaCl was 100 mmo·L－1 for Jinong 27 and Jinong 17; 75 mmo·L－1 for Jilin 30,DE2259 and Jinong 28. For soybean seedling the critical concentration of NaCl was 250 mmo·L－1for Jinong 27, Jinong 17, Jilin 30 and DE2259; 200 mmo·L－1 for Jinong 28.

References:

[1]王关林. 植物基因工程原理与技术[M]. 北京：科学出版社，1998：168-188. (Wang G L. Plant genetic engineering principles and technology[M]. Beijing: Science Press, 1998: 168-188.)

[2]Vasil I K. Molecular improvement of cereal[J]. Plant Molecular Biology, 1994, 25: 925-937.

[3]克拉克,著. 顾红雅，翟礼嘉，译. 植物分子生物学实验手册[M]. 北京：高等教育出版社，1998. (Clark M S, ed. Gu H Y, Qu L J, trans. Experimental plant molecular biology manual[M]. Beijing: Higher Education Press,1998.)

[4]Liu J F, Su Q, An J L, et al. Transfer of a minimal linear marker free and vector free?smGFP?cassette into soybean via ovary-drip transformation[J]. Biotechnology Letters, 2009, 31(2): 295-303.

[5]Yoder J L, Goldsbrough A P. Transformation systems for generating marker-free transgenic plants[J]. Biotechnology, 1994, 12: 263-257.

[6]贾士荣. 转基因植物食品中标记基因的安全性评价[J]. 中国农业科学， 1997，30(2)：1-15. (Jia S R. Safety evaluation of marker genes in transgenic food plants[J]. Scientia Agricultura Sinica, 1997, 30(2): 1-15.)

[7]于恒秀，刘巧泉，徐丽，等. 无抗性选择标记转基因软米和糯稻新品系的育及中间试验[J]. 作物学报，2009，35(6)：967-973. (Yu H X, Liu Q Q,Xu L, et al. Breeding and field performance of novel soft and waxy transgenic rice lines without selectable markers[J]. Acta Agronomica Sinica, 2009, 35(6): 967-973.)

[8]Rahman S M, Takagi Y, Kinoshita T. Genetic control of high stearic acid content in seed oil of two soybean mutants [J]. Theoretical and Applied Genetics, 1997, 95: 772-776.

[9]Romano E, Soares A, Proite K, et al. Transgene elimination in genetically modified dry bean and soybean lines[J]. Genetic Molecular Research, 2005, 4: 177-184.

[10]杨金慧，王丕武，曲静.?BADH/pepB双价基因无选择标记表达载体转化苜蓿的初步研究[J]. 中国草地学报，2009，31(4): 20-23. (Yang J H, Wang P W, Qu J, et al. Research on transforming BADH?and pepB?bivalent gene with marker free expression vector into Alfalfa[J]. Chinese Journal of Grassland, 2009, 31(4): 20-23.)

[11]邹莉，文艺，张匀华，等. 大豆子叶节对潮霉素敏感性研究[J]. 大豆科学，2008，27（2）：267-269.(Zou L, Wen Y, Zhang J H, et al. Susceptibility of soybean cotyledonary node to Hygromycin B[J]. Soybean Science, 2008, 27（2）：267-269.)

[12]袁鹰，刘德璞，王玉民，等. 卡那霉素对大豆生长的抑制及筛选试验研究[J]. 大豆科学，2003，22(4)：261-263. (Yuan Y, Liu D P, Wang Y M, et al. Study on critical and screen soybean grow of kanamycin[J]. Soybean Science, 2003, 22(4): 261-263.）

[13]张宁，司怀军，栗亮，等. 转甜菜碱醛脱氢酶基因马铃薯的抗旱耐盐性[J]. 作物学报，2009，35(6)：1146-1150. (Zhang N, Si H J, Li L, et al. Drought and salinity tolerance in transgenic potato expressing the betaine aldehyde dehydrogenase gene[J]. Acta Agronomica Sinca, 2009, 35(6): 1146-1150）

[14]刘玉杰，王宝增. 不同品种大豆耐盐性的比较研究[J]. 安徽农业科学， 2007，35(15)：4462-4464. (Liu Y J, Wang B Z. Comparison of the salt tolerlance in differentGlycine max?L.varieties[J]. Journal of Anhui Agricultural Sciences, 2007, 35(15): 4462-4464)

[15]刘海坤，卫志明.大豆遗传转化研究进展[J]. 植物生理与分子生物学学报，2005，31 (2)：126-134. (Liu H K, Wei Z M. Recent advances in soybean genetic transformation[J]. Acta Phytophysiologica Sinica, 2005, 31(2): 126-134.)

[16]Zubko E, Scutt C, Meyer P. Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes [J]. Nature Biotechnology, 2000, 18: 442-445.


Memo

Memo:
-
Last Update: 2014-09-12