Transformation of Phytase Gene into Soybean via Agrobacterium-mediated Method(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2011年03期

Page:

397-400

Research Field:

Publishing date:

Info

Title:

Transformation of Phytase Gene into Soybean via Agrobacterium-mediated Method

Author(s):

LIU Qi¹; XIA Shan-yong¹; ZHANG Jun²; DUAN Zhong-wei²; LIU Xin-lei³; LI Xi-chen¹

1. Biotechnology Research Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086;

2.Life Science College of Northeast Agricultural University，Harbin 150030;

3.Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China

Keywords:

Embryonic tips of soybean;  Agrobacterium;  Phytase gene

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2011.03.0397

Abstract:

The Phy gene cloned from Aspergillus awamori was transformed into soybean varieties Heinong37 and Jilinxiaoli soybean through Agrobacterium-mediated method with soybean embryonic tips as acceptors. The method has advantage of easy operation and lower chimera rate. The regenerated plants were screened by glufosinate-ammonium（1.0 mg·L^-1）of medium. The regenerated plantlets resistant to glufosinate-ammonium were detected by combining the PCR analysis of the target gene as well as selective marker gene and the herbicide screening. The result was intuitive and credible. Finally， 5 positive plants were obtained, and the transformation efficiency reached 0.5%.

References:

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