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Construction of Plant Expression Vector Involving HrpZpsta Gene and IIts Transformation into Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年03期
Page:
393-396
Research Field:
Publishing date:

Info

Title:
Construction of Plant Expression Vector Involving HrpZpsta Gene and IIts Transformation into Soybean
Author(s):
LI Bo;  FU Yong-ping;  WANG Pi-wu;  WANG Peng;  ZHANG Yun-yue; MA Jian
Biotechnology Center of Jilin Agricultural University, Changchun 130118, Jilin, China
Keywords:
Soybean;  hrpZpsta gene;  Vector construction;  Transgenic plants
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.03.0393
Abstract:
The hrpZpsta gene has broad disease-resistance and can improve the antiviral ability of plants. In this study, the hrpZpsta gene had been cloned from the cloning vector pMD18-T-hrpZpsta, and inserted into pBI121 to construct the vector pBI121-hrpZpsta. pBI121-hrpZpsta was transformed into cotyledon nodes of soybean Jinong 28 by ?Agrobacterium-mediated method. Kanamycin (100 mg·L－1) was applied to select the transformed tissue in selecting medium. Extracted DNA among the transgenic plants and analyzed it by PCR. The positive results indicated that hrpZpsta gene was successfully transformed into the genome of transgenic soybean.

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