|Table of Contents|

Molecular Identification of the Fungi in the Rhizospheric Soil of Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年03期
Page:
384-387
Research Field:
Publishing date:

Info

Title:
Molecular Identification of the Fungi in the Rhizospheric Soil of Soybean
Author(s):
JIE Wei-guang1;  ZHANG Yong1;  CAI Bai-yan2;  BAI Li1;  WANG Li-yang2;  LI Er-ping3
1.Department of Food and Environment Engineering, Heilongjiang East University, Harbin 150086, Heilongjiang;
2.Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, Heilongjiang;
3. College of Life Sciences, Northeast Forestry University, Harbin 150040, Heilongjiang, China
Keywords:
Soil;  Fungi;  Soybean;  Nested-PCR
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.03.0384
Abstract:
The fungi in the rhizospheric soil of soybean played a vital role in the formation of soil fertility, the spread of soil-borne diseases, the occurrence of allelopathy, etc. In particularly, soybean root rot caused by soil fungi has became a major disease which result in soybean production declined dramatically in China. Therefore, the rapid identification of the fungi in rhizospheric soil of soybean has great significance in preventing and curing fungal diseases of soybean. In this study, the fungal spores were isolated from soybean rhizospheres following a procedure including wet sieving, decanting, and separation in a sucrose gradient. Nested-PCR was conducted to specifically amplify the large-subunit (28S) rDNA D1/D2 domain sequences. They were identified from sequencing, and phylogenetic analysis. It showed that sequence alignment of the DT-1 28S rDNA D1/D2 domain sequence to fungi sequences in the database revealed that DT-1 shared 97%homology with the corresponding DT-1 sequence; DT-2 clusters shared 99%homology with the corresponding Mucor racemosus(Y213713) sequence. Results demonstrate that the method can get results quickly and accurately, and is suitable for identification of fungi in rhizospheric soil of soybean.

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Last Update: 2014-09-12