|Table of Contents|

Cloning and Analysis of Fd-GOGAT Genes from Different Soybean Varieties(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年03期
Page:
374-378
Research Field:
Publishing date:

Info

Title:
Cloning and Analysis of Fd-GOGAT Genes from Different Soybean Varieties
Author(s):
CHEN Li-hua1;  LIU Li-jun2;  LIU Ye-li2;  ZU Wei2
1.National Agriculture Standardize Supervise and Research Center, Harbin 150036；
2.Agronomy College, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
Keywords:
Soybean;  Fd-GOGAT genes;  Enrichment separation;  Clone
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.05.0374
Abstract:
Fd-GOGAT genes from six different soybean varieties were cloned. The comparative analysis showed the sequences of GOGAT genes in different soybean varieties were very identical, so we can design general real-time PCR primer to detect express regulation of Fd-GOGAT genes. The coding sequence of ?Fd-GOGAT gene in Dongnong42 appeared an absence of 8 nucleotides, which brought frameshift mutation，led to its C-terminal lack of 79 amimo acids conserved sequence and the GS1 big subunit C-terminal conserved domain（gltB_C）were not in their integrity. Molecular and protein evolution tree analysis showed Dongnong 42, Dongnong 46 and G.gracilis had similar evolution distance for Fd-GOGAT gene.

References:

[1]Frommer W B, Kwatr M, Hirner B,et al. Transporters for nitrogenous compounds in plants[J]. Plant Molecular Biology, 1994, 26:1651-1670.

[2]Chrispeels M J, Crawford N M, Schorede J L. Proteins for transport of water and mineral nutrients across the membrane of plant cells [J]. Plant Cell, 1999, 11:661-675.

[3]Cren M, Hirel B. Glulamine synthetase in higher plant: Regulation of gene and protein expression from the organ to the cell[J].?Plant Cell Physiology, 1999, 40:1187-1193.

[4]Lea P J, Blackwell R D, Joy K W.Ammonia assimilation in higher plants[M]// Meng K， Pilbeam D J.Nitrogen Melabotism of Plants. Oxford University Press, Newyork, 1992: 153-186.

[5]Refouvelet E, Daguin F. Polymorphic glutamate dehydrogenase inlilac vitro plants as revealed by combined preparative IEF and native PAGE: Effect of ammonium deprivation; darkness and atmospheric CO2 richment upon isomerization[J]. Physiologia Plantarum, 1999, 105:199-206.

[6]Melo-oliveira R, Oliveira I C, Coruzzi G M. Arabidppsis?mutant analysis and gene regulation define anonredundant role for glutamate dehydrogenase in nitrogen assimilation[J]. Proceedings of the National Academy of Sciences USA, 1996, 93: 4718-4723.

[7]Lea P J, Robinson S A, Stewart G R. The enzymology and metabolism of glutamine, glutamate, andasparagine[M]// Miflin B J，Lea P J. The Biochemistry of Plants. NewYork: Academic, 1990:121-159.

[8]Lea P J, Miflin B J. Alternative route for nitrogen in higher plants[J]. Nature, 1974, 251:614-616.

[9]Qusji G O, Madu W C. Ammonium ion salvage by glutamate dehyrogenase during defence response in maize[J]. Phytochemistry, 1996, 42: 1491-1498.

[10]刘丽君, 孙聪姝, 刘艳,等. 氮肥对大豆结瘤及叶片氮素积累的影响[J].东北农业大学学报, 2005, 36(2):133-137. (Liu L J, Sun C S, Liu Y, et al. Effects of nitrogen on nodule-forming and nitrogen concentration in soybean leaves[J]. Journal of Northeast Agricultural University, 2005, 36(2):133-137.)

[11]孙太靖, 龚振平, 马春梅. 大豆植株氮素积累与转运动态的研究[J].东北农业大学学报，2004, 35（5）：517-521. (Sun T J, Gong Z P, Ma C M. Study on the regulation of nitrogen accumulation and translocation in high-oil soybean[J]. Journal of Northeast Agricultural University, 2004, 35（5）：517-521.)

[12]代翠红,李杰,朱延明,等. 不同提取方法对四种重要作物提取效率的比较[J] . 东北农业大学学报, 2005，36(3):329-332.（Dai C H, Li J, Zhu Y M, et al. Comparison of the efficiency of different DNA extraction methods on 4 kinds of important crops[J ] . Journal of Northeast Agricultural University, 2005，36(3):329-332.）

[13]萨姆布鲁克.分子克隆实验指南（第三版）[M]. 北京：科学出版社, 2002:1-10.(Joseph sambrook. The condensed protocols from molecular cloning: a laboratory manual[M]. Beijing: Sciences Press, 2002: 1-10.)

[14]Suzuki A, Knaff D B. Glutamate synthase: structural, mechanistic and regulatory properties,and role in the amino acid metabolism[J]. Photosynthesis Research,2005,83: 191-217.

[15]Melo P M, Lima L M, Santos I M, et al. Expression of the plastid-located glutamine synthetase of Medicago truncatula. Accumulation of the precursor in root nodules reveals an in vivo control at the level of protein import into plastids[J]. Plant Physiology, 2003,132(1):390-399.


Memo

Memo:
-
Last Update: 2014-09-11