|Table of Contents|

Shoots Regeneration of Cotyledon Node from Soybean Immature Seeds(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年03期
Page:
369-373
Research Field:
Publishing date:

Info

Title:
Shoots Regeneration of Cotyledon Node from Soybean Immature Seeds
Author(s):
WANG Lu-ping QIAO Ya-ke LI Gui-lan ZHONG Lei CUI Shan-shan JI Zhan-bo
Life Science and Technology College, Hebei Normal University of Science and Technology, Changli 066600, Hebei, China
Keywords:
Soybean Immature seeds cotyledon node Induction
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.03.0369
Abstract:
Immature seed cotyledons nodes of Jidou 12 and Zhonghuang 13 were used as explants to research the effect of 6-BA concentration, time of obtaining materials and degree of seeds development on the proportion of shoots regeneration from immature seeds cotyledons nodes.The result showed that the best time of obtaining materials of Jidou 12 was 60-66 d after flowering, the optimal concentration of 6-BA in multiple shoots induction medium was 1.0-1.5 mg·L-1,and the proportion of shoots reached 96.67%-100%. The best time of obtaining materials of Zhonghuang 13 was 49-65 d after flowering, the optimal concentration of 6-BA in multiple shoots induction medium was 1.5 mg·L-1,and the proportion of shoots reached 93.33%-100%. Proportion of shoots by immature seeds cotyledons nodes were higher than mature seeds cotyledons nodes by 4%and 10%for Jidou 12 and Zhonghuang 13, respectively.

References:

[1]Hinchee M A W, Connor-Ward D V, Newell C A, et al. Production of transgenic soybean plants using Agrobacterium -mediated DNA transfer[J]. Nature Biotechnology, 1988, 6: 915-922.

[2]McCabe D E, Swain W F, Martinell B J, et al. Stable transformation of soybean (Glycine max) by particle acceleration[J]. Nature Biotechnology, 1988, 6: 923-926.

[3]Dhir S K, Dhir S, Savka M A, et al. Regeneration of transgenic soybean(Glycine max) plants from electroporated protoplasts[J]. Plant Physiology, 1992, 99: 81-88.

[4]卜云萍, 李明春, 胡国武, . 大豆子叶节组培再生系统与农杆菌介导的基因转化系统的比较研究[J]. 南开大学学报(自然科学版), 2003, 36 (1): 103-108. (Pu Y P, Li M C, Hu G W, et al. The study of comparing the transformation system of Agrobacterium-mediated and regeneration system of cotyledon nod of soybean culture[J]. Acta Scientiarum Naturalium Universitatis Nankaiensis(Natural Science Edition), 2003, 36 (1): 103-108.)

[5]Tsai Y C, Hitoshi S. Plant regeneration from soybean cotyledonary node segments in culture[J].Plant Science Letters, 1980, 19: 91-99.

[6]Kartha K K, Pahl K, Leung N L, et al. Plant regeneration from meristems of grain legumes: soybean, cowpean, peanut, chickpea and bean[J]. Canadian Journal of Botany, 1981, 59: 1671-1679.

[7]Barwale U B, Kems H R, Widholm J M. Plant regeneration from callus cultures of several soybean genotypes via embryo gensis and organogenesis[J]. Planta, 1986, 167: 473-481.

[8]Wright M S, Ward D V, Hinchee M A, et al. Regeneration of soybean(Glycine max?L. Merr)from cultured primary leaf tissue[J]. Plant cell Reports, 1987, 6: 83-89.

[9]Seth M, Ralph S, John C. A simple rapid protocol for adventitious shoot development from mature cotyledons of(Glycine max)cv Bragg[J]. In Vitro Cellular and Devepmental Biology, 1989, 25(4): 385-388.

[10]Liu H K, Yang C,Wei Z M. Efficient Agrobacterium tumefaciens-mediated transformation of soybeans using an embryonic tip regeneration system[J]. Planta, 2004, 219: 1042-1049.

[11]Ko T S, Lee S, Farrand S K, et al. A partially disarmed vir helper plasmid, pKYRT1, in conjunction with 2, 4-dichlorophenoxyacetic acid promotes emergence of regenerable transgenic somatic embryos from immature cotyledons of soybean[J]. Planta, 2004, 218: 536-541.

[12]Ko T S, Lee S, Krasnyanski S, et al. Two critical factors are required for efficient transformation of multiple soybean cultivars: Agrobacterium?strain and orientation of immature cotyledonary explant[J]. Theoretical and Applied Genetics, 2003, 107: 439-447.

[13]Finer J J, Nagasawa A. Development of an embryogenic suspension culture of soybean [Glycine max(L.) Merrill][J]. Plant Cell, Tissue and Organ Culture, 1988, 15: 125-136.

[14]Meurer C A, Dinkins R D, Redmond C T, et al. Embryogenic response of multiple soybean cultivars across three locations[J]. In Vitro Cellular Developmental Biology-Plant, 2001, 37: 62-67.

[15]Samoylov V M, Tucker D M, Thibaud-Nissen F, et al. A liquid-medium-based protocol for rapid regeneration from embryogenic soybean cultures[J]. Plant Cell Reports, 1998, 18: 49-54.

[16]Samoylov V M, Tucker D M, Parrott W A. Soybean embryogenic cultures: the role of sucrose and total nitrogen content on proliferation[J]. In Vitro Cellular Developmental Biology-Plant, 1998, 34:8-13.

[17]Walker D R, Parrott W A. Effect of polyethylene glycol and sugar alcohols on soybean somatic[J]. Plant Cell, Tissue and Organ Culture, 2001, 64(1): 55-62.

[18]Lazzeri P A, Hidebrand D F,Collins G B. A procedure for plant regeneration from immature cotyledon tissue of soybean[J]. Plant Molecular Biology Reporter, 1987, 10: 197-208.

[19]李文霞, 宁海龙, 李文滨, . 6-BA对大豆子叶节再生的影响[J]. 核农学报, 2007, 21(5): 502-505. (Li W X, Ning H L, Li W B, et al. Effect of 6-BA on regeneration of soybean cotyledonary node[J]. Journal of Nuclear Agricultural Sciences, 2007, 21(5): 502-505.

[20]龚学臣, 季静, 王萍, . 苗龄与6-BA浓度对大豆子叶节丛生芽诱导的影响. 吉林农业大学学报[J], 2005, 27 (2): 128-130.(Gong X C, Ji J, Wang P, et al. Effect of aseptic seeding age and 6-BA concentration on overgrowing shoot s of soybean cotyledonary node[J]. Journal of Jilin Agricultural University[J], 2005, 27 (2): 128-130.)

[21]李桂兰, 乔亚科, 杨少辉, . 农杆菌介导大豆子叶节遗传转化的研究[J]. 作物学报, 2005, 31(2): 170-176. (Li G L, Qiao Y K, Yang S H, et al. Study of the Agrobacterium-mediated transformation systems of soybean cotyledonary node[J].Acta Agronomica Sinica, 2005, 31(2): 170-176.)

[22]王萍, 吴颖, 杨武杰, . 大豆未成熟子叶体细胞胚胎发生及其相关因子的分析[J]. 中国油料作物学报, 2002, 24(1): 29-31. (Wang P, Wu Y, Yang W J, et al. Somatic embryogenesis from immature cotyledons of soybean and analysis of correlative factors[J]. Chinese Journal of Oil Crop Sciences, 2002, 24(1): 29-31.)

[23]吴超, 武天龙. 大豆未成熟子叶体细胞再生及相关农艺性状影响因子的分析[J].大豆科学, 2004, 23(1): 21-24. (Wu C, Wu T L. Somatic embtyogenesis from immature cotyledons and analysis of correlative agronomic characters[J]. Soybean Science, 2004, 23(1): 21-24.)


Memo

Memo:
-
Last Update: 2014-09-11