|Table of Contents|

Technique flow and Key Operation Points of Agrobacterium-mediated Genetic Transformation of Soybean Cotyledonary Node(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年03期
Page:
362-368
Research Field:
Publishing date:

Info

Title:
Technique flow and Key Operation Points of Agrobacterium-mediated Genetic Transformation of Soybean Cotyledonary Node
Author(s):
ZHAO Xiao-wen WU Fang-fang DI Shao-kang BAI Xi JI Wei LI Yong CAI Hua ZHU Yan-ming
College of Life Science, Northeast Agicultural University, Harbin 150030, Heilongjiang, China
Keywords:
Soybean Genetic transformation Agrobacterium-mediated Cotyledonary node Technique flow
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.03.0362
Abstract:
The low soybean genetic transformation frequency was one of the difficult problems that needed be resolved in soybean transgenic breeding. In this study, the soybean cotyledonary node was used as explants, and the technique flow and key operation points of Agrobacterium-mediated transformation method, including Agrobacterium preparation, explant and aseptic seedling preparation, infection and co-cultivation, adventitious bud induction, shoot elongation, regeneration of transgenic plantlets, plant habituation, obtaining of antibiotic resistant detection of transgenic plants, was systematically investigated. Therefore, a stable and efficient genetic transformation system using soybean cotyledonary nodes as explants was established. Using this transformation system, ?A1 gene isolated from Glycine soja was transformed into four soybean cultivars, including Suinong28, Hefeng55, Hefeng50 and Dongnong50. And several important factors affecting the transformation efficiency were also discussed in the explant.

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Last Update: 2014-09-11