|Table of Contents|

Transgenic Soybean (Glycine max. L） Plants with phyA Gene Improved Phytase Activity(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年03期
Page:
356-361
Research Field:
Publishing date:

Info

Title:
Transgenic Soybean (Glycine max. L） Plants with phyA Gene Improved Phytase Activity
Author(s):
YAN Rui-ye1;  LI Xi-huan1;  LI Gui-lan2;  CHANG Wen-suo1;  ZHANG Cai-ying1
1. North China Key Laboratory of Crop Germplasm Resources, Education Ministry of China, Agricultural University of Hebei, Baoding 071001;
2. Life Science and Technology College, Hebei Normal University of Science & Technology, Changli 066600, Hebei, China
Keywords:
Soybean;  Phytase gene;  Genetic transformation; Agrobacterium-mediated
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.03.0356
Abstract:
Organic phosphorus (P) is the main form of phosphorus in soil, of which the phytate accounted for more than 50%. The available way that improves plant P nutrition is to hydrolyze phytate into inorganic phosphorus (Pi) to increase the soil P utilization efficiency. In this study, a phytase gene (phyA), driven by the root-specific pyk10 promoter and directed by a carrot extracellular targeting peptide, was introduced into different soybean varieties (Jidou 12, Jidou 16, Wuxing 1 and Jilin 35） by Agrobacterium-mediated and pollen tube pathway transformation method. PCR analysis results showed that the phyA was successfully integrated into the soybean genome. Total 114, 101 and 28 PCR positive plants were obtained from T0, T1 and T2 generation, respectively. The results also showed that the T4 generation plants could grow better than wild-type when cultured under the condition in which phytate was the sole source of phosphorus. And the secreted phytase activities of roots also indicated that three transgenic lines were increased by 5%, 13% and 24% compared to the wild-type plants, respectively.

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