|Table of Contents|

Clone and Analysis of Thioredoxin Gene from Soybean (Glycine max L.)(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年03期
Page:
351-355
Research Field:
Publishing date:

Info

Title:
Clone and Analysis of Thioredoxin Gene from Soybean (Glycine max L.)
Author(s):
WANG Wei-qi;  HOU Wen-sheng
National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Keywords:
Soybean;  Salt;  Thioredoxin gene;  Gene clone
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.03.0351
Abstract:
Based on screening the suppression subtractive hybridization library which constructed by soybean cultivar ‘wenfeng7’ (salt-tolerant) after salt treatment, we obtained some differentially expressed EST sequences. The EST sequences were compared with the NCBI databases, analysis result showed that one EST sequence related to thioredoxin. A novel thioredoxin gene (Trx) of Glycine max was cloned and identified with the EST obtained from suppression subtractive hybridization library. Bioinformatics analysis showed that the full length of the GmTrx gene cDNA was 354 bp, coded 118 amino acids. Compared with several plants, this gene showed 72%and 76%homology to thioredoxin protein in Arabidopsisand tobacco, respectively. The expression of different soybean varieties under salt and common treatment was analyzed by Real-time PCR method. The result showed that, the expression of Trx gene was up-regulated in both salt-tolerant and sensitive soybean cultivars after salt treatment, although the expression in salt-tolerant cultivars was much higher than that in salt-sensitive cultivars. This gene may be very important for improving the salt tolerance of soybean.

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