Research on Cotyledonary Nodes Regeneration System of Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2011年02期

Page:

303-305

Research Field:

Publishing date:

Info

Title:

Research on Cotyledonary Nodes Regeneration System of Soybean

Author(s):

LAI Bing-bing¹;  HAN Yang¹; 2; 3;  LI Chun-feng¹;  ZHANG Xing-zheng¹;  ZHANG Li-jun²;  SONG Shu-hong³

1.School of Life Science, Liaoning University, Shenyang 110036；

2.College of Biological Sciences and Technology，Shenyang Agricultural University，Shenyang 110866；

3.Crop Institute, Liaoning Academy of Agricultural Science，Shenyang 110866， Liaoning， China)

Keywords:

Soybean; Cotyledonary node; Adventive shoots; Plant Regeneration

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2011.02.0303

Abstract:

The adventive shoots were induced from cotyledon nodes with soybean cultivar Liaodou 17, Liaodou 18 and Liaodou 23 as explants. With the increase of 6-BA concentration, the number of adventitious shoots on each explant showed an increasing trend, when 0.05 m·L^-1 IBA and 4.0 m·L^-1 6-BA were added into 1/2MSB medium, the induction rate of adventive shoots is the highest. Compared the effect of explants, the explants of reserving half cotyledon was better than the cotyledon nodes without cotyledon. Therefore, the optimum culture medium for elongation of adventitious bud was 1/2MSB+0.5 m·L^-1 6-BA+0.05 m·L^-1 IBA, and the optimum culture medium for rooting was 1/2MSB+1.0 mg·L^-1 IBA.

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