Screening and Molecular Identification of Bacteria Induced Anti-adversity Effect in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2011年02期

Page:

205-210

Research Field:

Publishing date:

Info

Title:

Screening and Molecular Identification of Bacteria Induced Anti-adversity Effect in Soybean

Author(s):

HUANG Shan-shan¹; DUAN Yu-xi¹; CHEN Li-jie¹; WANG Yuan-yuan²; SHEN Hong-qiu¹; WAN Chuan-hao¹; CHEN Jing-sheng³; CUI Shao-bin⁴

1.Department of Plant Protection，Shenyang Agricultural University，Shenyang 110161，Liaoning；

2.Department of Biological Science and Technology，Shenyang Agricultural University，Shenyang 110161，Liaoning；

3.Daqing Branch，Heilongjiang Academy of Agricultural Sciences，Daqing 163316，Heilongjiang；

4. Heihe Branch， Heilongjiang Academy of Agricultural Sciences，Heihe 164300，Heilongjiang，China

Keywords:

Bacteria; Heterodera glycines; Root rot; Induced resistance; 16SrDNA

PACS:

S 565.1

DOI:

10.11861/j.issn.1000-9841.2011.02.0205

Abstract:

To provide theoretic base for screening of bacteria induced anti-adversity effect from bacteria induced cold resistance in soybean，soybean seeds coated by bacteria were grown on farmland where Heterodera glycines occurred seriously in Liaoning and Heilongjiang province with liquid nutrient medium treatment as control，and then investigated the growth of soybean seedlings and the cyst nematode occurrence, and performed molecular identification of outstanding strains. Sneb69, Sneb82, Sneb179 and Sneb218 showed significant cold resistant effect for soybean seedlings. It was also showed that bacteria could control soybean cyst effectively as the treatment of soybean coated by strain Sneb69,Sneb82,Sneb179and Sneb218 showed soybean cyst control effect by analyzing the number of cyst on the root, the number of cyst in the soil and the number of soybean cyst nematodes in the root. In different district, bacteria treatments showed stable resistance to adverse environment, it proved Sneb69，Sneb82，Sneb179and Sneb218 would become the potential agents to resist adverse environment. Sneb82 and Sneb218 showed inhibitory effect on root rot. Six physiological indicators of soybean under bacteria strain showed better performace compared with the control, indicating strain Sneb69, Sneb82, Sneb179 and Sneb218 had good adversity tolerance ability. 16SrDNA sequence analysis showed Sneb69，Sneb82 and Sneb218 were related to Bacillus megaterium and Sneb179 were related to Pseudomonas aeruginosa.

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