|Table of Contents|

Cloning and Expression Analysis of Glycine max γ-tocopherol Methyltransferase Gene(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年02期
Page:
198-204
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of Glycine max γ-tocopherol Methyltransferase Gene
Author(s):
HU Ying-kao;  MENG Xian-ping;  LI Ya-xuan;  CAI Min-hua
College of Life Science, Capital Normal University, Beijing 100048，China
Keywords:
Glycine max;  γ-tocopherol methyltransferase;  Gene cloning;  in silico expression analysis
PACS:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2011.02.0198
Abstract:
cDNA sequence of γ-tocopherol methyltransferase gene was cloned by using in silico cloning combined with experimental RT-PCR from Glycine max with a GenBank accession number AY960126. Nucleotide sequence analysis showed that the cDNA has an intact open reading frame (ORF) encoding 350 amino acids. Two same frame stop codons were found in 5′ untranslated region and two tailing signals and a polyA tail were found in 3′ region. Many light responsive elements were found in its promoter region including many common core promoter elements. The deduced protein contained a signal peptide located in chloroplast and two γ-tocopherol methyltransferase motifs. Protein multiple-alignment and phylogenetic analysis suggested that GmTMT was strong similarity in different plant species. Expression analysis with in silico and RT-PCR results showed that its expression was closely correlated with chloroplast content. However, high light stress has no effect on its expression.

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Last Update: 2014-09-11