|Table of Contents|

Boric Acid Mediated Transformation of Soybean (Glycine max) Cotyledonary Node(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年02期
Page:
194-197,204
Research Field:
Publishing date:

Info

Title:
Boric Acid Mediated Transformation of Soybean (Glycine max) Cotyledonary Node
Author(s):
LI Hai-fen1; LIU Qing-mei1; YNAG Guang-yu2; WANG Yang2; YUE Na1; HU Qian-qian1; MENG Xiang-xun1
1.School of Basic Medicine and Biological Science, Soochow University, Suzhou 215123，Jiangsu;?
2.Soybean Insititute, Jilin Academy of Agricultural Sciences, Changchun 130033，Jilin, China
Keywords:
Soybean; Transgene; Agrobacterium; Boron acid; Multiple shoots
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.02.0194
Abstract:
The agrobacterium-mediated transgenic technique combined with the treatment of boric acid was studied for the transformation of foreign gene Bt to soybean cotyledon nodes. The results showed that shoots regenerated from the explants were multiplied best at 200 mg·L-1 of boric acid concentration, and the transformation ratio reached 3.3%. Regenerated seedlings were screened by genomic PCR amplification and two seedlings on inductive medium of 200 mg·L-1 boron acid was obtained and proved to be positive by reverse PCR and Southern blotting. It was demonstrated that the target gene had been transformed into the soybean genome.

References:

[1]潘川芝,李凤,戴良英.大豆子叶节离体再生体系优化研究[J].湖南农业科学,2006（5）:31-36.(Pan C Z, Li F, Dai L Y. Regeneration system of soybean cotyledonary nodes[J].Hunan Agricultural Sciences, 2006（5）:31-36.)

[2]Loomis W D, Durst R Y. Chemistry and biology of boron[J].BioFoctors,1992,3:229-239.

[3]施益华,刘鹏.硼在植物体内生理功能研究进展 [J]. 亚热带植物科学,2002,3l(2):64-69.(Shi Y H, Liu P. A review of advances in physiological function of boron in plants[J]. Subtropical Plant Science,2002,3l(2):64-69.)

[4]葛正珍,杨光宇，仲艳，等.硼对大豆愈伤组织的细胞结构及形态的影响[J].大豆科学,2009,28（2）:225-228.（Ge Z Z，Yang G Y, Zhong Y, et al. Effect of boron on cell structure and shape in soybean(Glycine max)callus[J]. Soybean Science,2009,28(2):225-228.）

[5]李凤.大豆子叶节高效再生体系的建立与GmCOII基因的遗传转化[D].长沙:湖南农业大学,2006.(Li F. Establishment of effective regeneration system of soybean cotyledonary nodes and genetic transformation of GmCOII?gene in soybean[D].Changsha：Hunan Agricultural University, 2006.)

[6]Annette D, Giancarlo P, Maria H, Integrated bombardment and agrobacterium transformation system: an alternative method for soybean transformation[J]. Plant Molecular Biology Reporter, 2000,18:51-59.

[7]Olhoft P M, Flagel L E, Donovan C M, et al. Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method[J].Planta,2003,216: 723-735.

[8]邱承祥.大豆茎尖再生系统的研究及农杆菌介导的Bt基因的转化[D].上海：上海交通大学,2003.（Qiu C X. Study on shoot tip regeneration system of soybean and agrobacteriun-mediated bt gene transformation[D]. Shanghai: Shanghai Jiaotong University,2003.）

[9]Xue R G, Xie H F, Zhang B. A multi-needle-assasted transformation of soybean cotyledonary node cells[J].Biotechnology Letters, 2006,28:1551-1557.

[10]Margie M P, Juan C M, Andrea B K, et al. Improved cotyledonary node method using an alternative explant derived from mature seed for efficient Agrobacterium-mediated soybean transformation[J]. Plant Cell Reports,2006, 25: 206-213.

[11]Margie M P, Shou H X,Guo Z B, et al.Assessment of conditions affecting agrobacterium-mediated soybean transformation using the cotyledonary node explant[J].Euphytica,2004, 136: 167-179.

[12]吴静,李春俭,江荣风,等.缺硼对大豆幼苗形成和根瘤固氮活性的影响[J].华北农学报,1999,14（2）：96-101. (Wu J,Li C J, Jiang R F, et a1.Effects of boron-deficiency on soybean growth and nitrogenase activity[J].Acta Agricuhurae Boreali-Sinica,1999,14(2):96-101.)

[13]屈红征,王丽萍,吴国良.植物硼素营养研究进展[J].山西农业大学学报,2001,21（2）:173-176.(Qu H Z, Wang L P, Wu G L. Progress of the study on boron nutrition of plant[J]. Journal of Shangxi Agricultural University, 2001,21（2）:173-176.)

[14]刘鹏,杨玉爱.钼、硼浸种对大豆幼苗生理特性的影响[J].浙江大学学报,2003,30（1）:83-88.(Liu P, Yang Y A. Effect of soaking of molybdenum and boron on physiological characteristics of soybean seedling[J]. Journal of Zhejiang University, 2003,30（1）:83-88.)

[15]黄益宗.植物对硼素不足的反应及其成因探讨[J].土壤与环境,2002,11（4）:434-438.(Huang Y Z. Plant response to boron deficiency and origin of formation[J]. Soil and Environmental Sciences,2002,11（4）:434-438.)

[16]石磊,徐芳森.植物硼营养研究的重要进展与展望[J].植物学通报, 2007, 24 (6): 789-798.(Shi L，Xu F S. Main progresses in boron nutrition research in plants and its prospects [J].Chinese Bulletin of Botany, 2007, 24 (6): 789-798.)

[17]Takano J P, Kyoko M W, Toru F W. Boron transport mechanisms: collaboration of channels and transporters[J].Trends in Plant Science,2008, 13(8):451-457.

[18]邓向阳,卫志明.大豆转化技术[J].植物生理学通讯,1998,34(5):381-387.（Deng X Y, Wei Z M. Technique of transformation of soybean[J]. Plant Physiology Communications,1998,34(5):381-387.）

[19]Meurer C A, Dinkins R D, Collins G B. Factors affecting soybean cotyledonary node transformation[J].Plant Cell Reports,1998,18:180-186.


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Last Update: 2014-09-11