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Establishment of an Optimized System about Extraction-Determination of Soybean Isoflavone(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年01期
Page:
141-143,145
Research Field:
Publishing date:

Info

Title:
Establishment of an Optimized System about Extraction-Determination of Soybean Isoflavone
Author(s):
ZHANG Yan; LI Xiao-wei; ZHANG Hai-jun; ZHANG Qing-lin; ZHAI Ying; QIAN Dan-dan; YAN Xiao-feng; WANG Qing-yu; LI Jing-wen
College of Plant Science, Jilin University, Changchun 130062, Jilin, China
Keywords:
Soybean isoflavones;  Ultrasonic wave;  Three-wave length UV spectrohotometry;  Optimized system
PACS:
TS201.1
DOI:
10.11861/j.issn.1000-9841.2011.01.0141
Abstract:
The method to extract soybean isoflavone by ultrasonic wave was optimized, the content of which was determined by three-wave length UV spectrohotometry, in order to develop a high performance extraction-determination method of isoflavones in soybean. Results showed that the optimized condition were as follows: the ratio of material to solvent was 1∶20,concentration of ethanol was 50%,extraction time was 30 min,with 2 replicates. Twenty-four soybean varieties was mesured, their contents varied significantly. The content of soybean isoflavones was highest in Jilin 32 and Jilin 35,0.292% and 0.259%， respectively.

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Last Update: 2014-09-11