|Table of Contents|

Preparing and Digesting Megabase-size Nuclear DNA of Glycine soja Genome(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年01期
Page:
33-36,40
Research Field:
Publishing date:

Info

Title:
Preparing and Digesting Megabase-size Nuclear DNA of Glycine soja Genome
Author(s):
LI Xiao-ling12 LI Ke-xiu2 ZHAO Hong-kun1 DONG Ying-shan1
1.Biotechnology Research Centre, Jinlin Academy of Agricultural Sciences, Changchun 130033;
2.School of Chemistry and Life Science Changchun University of Technology, Changchun 130012, Jilin, China
Keywords:
Glycine sojaMegabase-size DNA Pulsed field gel electrophoresis TAC Genomic library
PACS:
Q781
DOI:
10.11861/j.issn.1000-9841.2011.01.0033
Abstract:
Nuclear DNA was extracted from etiolated seedlings of Glycine soja. Generally, the nuclei were embeded in low-melting-point agarose pulgs, digested with proteinase K and depurated with pulsed field gel electrophoresis to yield about two-Megabase-size DNA. The concentration of DNA solution could be up to 10 ng·μL-1 after partial digestion by HindⅢ, elution by pulsed field gel electrophoresis,concentration and dialysis. The result of examination by ligation and eletroporation showed that the DNA obtained by the method was suitable for consequent construction of genomic library of Glycine soja.

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Last Update: 2014-09-11