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Mapping and Structure Analysis of Key Enzyme Genes in Anthocyanin Synthesis of Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年01期
Page:
24-28,32
Research Field:
Publishing date:

Info

Title:
Mapping and Structure Analysis of Key Enzyme Genes in Anthocyanin Synthesis of Soybean
Author(s):
ZHU Xiao-shuang1;  LIU Chun-yan2;  YANG Zhen1;  JIANG Hong-wei2;  CHEN Qing-shan1;  HU Guo-hua2; 3
1. College of Agriculture, Northeast Agricultural University, Harbin 150030;
2. The Crop Research and Breeding Center of Land-Reclamation, Harbin 150090;
3. The National Research Center of Soybean Engineering and Technology, Harbin 150050, Heilongjiang, China
Keywords:
Soybean;  Anthocyanin;  Gene;  Mapping;  Gene structure
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.01.0024
Abstract:
Seedcoat mottling would be induced in soybean when infected by soybean mosaic virus, which could be greatly decrease the economic value. Seedcoat mottling of soybean was due to the abnormal accumulation of flavonoid. And anthocyanin belongs to flavonoid was a kind of major secondary metabolites. Sixteen genes of key enzyme in anthocyanin synthesis, including PAL, chalcone synthase (9 members of CHS family), F3H, CHI, DFR, and 4CL, were mapped on the soybean linkage groups, based on the soybean genome sequence and marker information, and their gene structures were analyzed. The results showed that 16 genes were mapped on ten linkage groups, including A1, A2, B1, B2, D1a, D1b, D2, I, K, and O, and the flank markers of the gene on the linkage groups were obtained. Furthermore, the sequence information between cDNA and gDNA was compared, the number of exon was from 1 to 7, and the number of intron was from 0 to 6. Among them, PAL, DFR2, and GmCHS7 were all single-extron gene, but there were one intron in 4CL, CHI, F3H, GmCHS1, GmCHS5, and GmCHS8, two introns in DFR1, GmCHS2, GmCHS3, and GmCHS6, three introns in GmCHS4 and GmCHS9, and six introns in GmIRCHS. The corresponding markers obtained from the mapping were available for molecular assisted selection, while the structure information could be better used in gene function analysis.

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