|Table of Contents|

Optimization of Biolistics Transformation of Embryonic Tips of Soybean ［Glycine max (L.) Merrill］ Mature Seeds(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年01期
Page:
20-23
Research Field:
Publishing date:

Info

Title:
Optimization of Biolistics Transformation of Embryonic Tips of Soybean ［Glycine max (L.) Merrill］ Mature Seeds
Author(s):
YE Mei1;  ZHANG Min1;  YANG Su-xin1; SHAO Qun1;  AN Hai-long2;  CAO Zi-yi1;  FENG Xian-zhong1
1. Key Laboratory of Plant Stress Research of Shandong Province, College of Life Science, Shandong Normal University, Jinan 250014;
2. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an 271018, Shandong, China
Keywords:
Glycine max;  Embryonic tips of mature seeds;  Biolistics;  Genetic transformation
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.01.0020
Abstract:
To establish an efficient transformation system of embryonic tips of soybean mature seeds, effects of 6-BA and hygromycin B concentration were studied during biolistics transformation of soybean ［Glycine max (L.) Merrill cv ‘Hedou12’］. The UidA (GUS) gene was employed as a reporter gene to reveal the foreign gene expression and integration. Both number and elongation length of multiple shoot reached the highest values at 5 mg·L-1 6-BA for 16 h induction of embryonic tips of mature seeds. The optimal selection scheme of transformants was a gradient concentration of hygromycin B from 5 mg·L-1 to 15 mg·L-1. The expression of GUS gene was observed in both embryonic tips after particle bombardment and young leaves of progeny transformants. The integration of GUS gene into the soybean genome was demonstrated by PCR and sequencing analysis.

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