|Table of Contents|

Comparison on Methods Screening of Transgenic Soybean Resistant to Glufosinate(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年06期
Page:
1019-1023
Research Field:
Publishing date:

Info

Title:
Comparison on Methods Screening of Transgenic Soybean Resistant to Glufosinate
Author(s):
SUN Lei1; 2; HAN Jun-you1; LI Hong-yu2
1.College of Plant Science, Jilin University, Changchun 130000， Jilin;
2.Institute of Crop Sciences， Chinese Academy of Agricultural Sciences, Beijing 10081,China
Keywords:
Soybean; Regeneration; Screening; Glufosinate
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.06.1019
Abstract:
Four screening methods and several screening concentrations were used to screen soybean regeneration plant and the resistance of its progeny as well as to resolve the problem of high false-positive regeneration plants in agrobacterium-mediated transformation of soybean cotyledons after glufosinate screening. After screening the 6802 transgenic plants by spraying the leaf, 9 resistant plants were obtained, and the molecular detection of their progeny showed that they were all positive transgenic plants. Therefore, the results indicated that the method that screening the transgenic plant by the leaf spraying was feasible.

References:

[1]余永亮， 梁慧珍， 王树峰， 等.中国转基因大豆的研究进展及其产业化[J].大豆科学，2010，29(1)：143-150. （Yu Y L, Liang H Z, Wang S F, et al. Research progress and commercialization on transgenic soybean in China[J].Soybean Science，2010，29(1)：143-150.）

[2]盛东峰， 杨同文. 转基因大豆的研究及应用[J].周口师范学院学报，2007，25(2)：83-85.（Sheng D F，Yang T W. Transgenic soybean research and security assessment[J].Journal of Zhoukou Normal University, 2007，25(2)：83-85.）

[3]Vain P, McMullen M D, Finer J J. Osmotic treatment enhances particle bombardment-mediated transient and stable transformation of maize[J].Plant Cell Reprots,1993,12:84-88.

[4]Chen S Y. High-efficiency agrobacterium-mediated transformation of soybean [J]. Acta Botanica Sinica, 2004, 46(5):610-617.

[5]薛仁镐,谢宏峰. 利用改良的草铵膦筛选系统快速而有效筛选转基因大豆[J]. 大豆科学，2006, 25(4):373-378.(Xue R G, Xie H F. Rapid and efficient selection for transgenic soybean plants with the improved glufosinate selection system[J].Soybean Science, 2006, 25(4):373-378.)

[6]唐晓飞, 刘丽君, 张小明, 等. 高产大豆新品系哈交5337和哈交5489再生条件的优化[J]. 大豆科学，2008，27(4):203-207. （Tang X F, Liu L J, Zhang X M, et al. Improvement of regeneration system in high yield soybean lines Hajiao5337 and Hajiao5489[J]. Soybean Science，2008, 27(4):203-207.）

[7]寇坤， 刘丽君， 曲姗姗， 等.大豆新品系黑农56子叶节再生体系的优化[J].大豆科学，2009，28(3)：400-408.（Kou K，Liu L J, Zhang X M, et al. Improvement of regeneration system in new soybean line Heinong 56[J]. Soybean Science，2009, 28(3):400-408.）

[8]刘敏， 朱洪德， 高中利.抗除草剂转基因大豆遗传分析[J].大豆科学，2010，29(1)：33-36.（Liu M, Zhu H D,Gao Z L.Inheritance analysis of herbicide-resistant transgenic soybean[J].Soybean Science，2010，29(1)：33-36.）

[9]刘晓冰， Stephen J．Herbert, 金剑, 等.增加光照及其与改变源库互作对大豆产量构成因素的影响[J].大豆科学，2006，25(1)：6-10.（Liu X B，Herbert S J，Jin J, et al. Light enrichement and its interactions with source-sink alteration on yield and yield components in soybean[J]. Soybean Science,2006,25(1):6-10.）

[10]应珊， 何晓薇， 王秀荣， 等.影响农杆菌介导的大豆转化效率的因素研究[J].分子植物育种，2008，6(1)：32-40.（Ying S，He X W，Wang X R，et al. Assessment of factors affecting the transformation efficiency of soybean cotyledonary-node agrobacterium-mediated transformation system[J].Molecular Plant Breeding,2008,6(1):32-40.）

[11]宋雯雯, 刘宝辉, 杨明亮, 等. 农杆菌介导法将SPS基因导入大豆的研究[J].大豆科学，2008，27(3):387-390. （Song W W，Liu B H, Yang M L, et al. Transformation of SPS gene into soybean via agrobacterium-mediated method[J]. Soybean Science, 2008，27(3):387-390.）

[12]王庆中. 转BoTMT基因大豆的获得及鉴定[D].北京：中国农业科学院，2006：20-23. （Wang Q Z. Obtaining and identification of transgenic soybean with the gene of BoTMT[D]. Beijing: Chinese Academy of Agricultural Sciences,2006:20-23.）


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Last Update: 2014-09-15