|Table of Contents|

Gliadin Genetic Diversity in Soybean Varieties in Heilongjiang Province(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年06期
Page:
934-937
Research Field:
Publishing date:

Info

Title:
Gliadin Genetic Diversity in Soybean Varieties in Heilongjiang Province
Author(s):
SHA Wei; HE Hai-yan;  ZHANG Yan-fu
College of Life Science and Forestry, Qiqihar University, Qiqihar 161006,Heilongjiang，China
Keywords:
Soybean; Gliadin; Genetic diversity; Cluster analysis; Principal component analysis
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.06.0934
Abstract:
The SDS polyacrylamide gel eleetrophoresis(SDS-PAGE) was used to detect the gliadin genetic diversity of 25 soybean varieties in Heilongjiang province, and the results were as follows: 1) A total of 19 bands were detected in 25 cultivars, the polymorphic bands were 12, amount to 63.16％, among α, β, γ and ε zones，there were 3, 5, 5 and 6 bands, respectively. The Nei’s genetic similarity coefficient of the tested cultivars ranged from 0.4737 to 0.9474, and the average GS was 0.7105;the genetic distances of the tested cultivars ranged from 0.0541 to 0.7472, the average GD was 0.3654, these results suggested that there was a rich genetic polymorphism of gliadin among the cultivars. 2) According to the cluster analysis, the tested cultivars could be classified into 4 groups at the level GS 0.79, and principal component analysis verified the results further. Thus, gliadin clusters markers could efficiently classify the soybean varieties, and laid the foundation for soybean variety identification and the utilize of germplasm resources.

References:

[1]钱迎倩，马克平．生物多样性研究的原理与方法[M]．北京：中国科学技术出版社，1994：141-165．(Qian Y Q,Ma K P． The principle and method for Biodiversity research [M]．Beijing: Science and Technology Press，1994：141-165．)

[2]马啸，陈仕勇，周永红，等．老芒麦种质的醇溶蛋白遗传多样性研究[J]．草业学报2009,18（3）： 59-66．(Ma X, Chen S Y, Zhou Y H, et al．Genetic diversity of Gliadin in worldwide germplasm collections of Elymus sibiricus[J]．Acta Prataculturae Sinica， 2009,18（3）：59-66．)

[3]张学勇，杨欣明，董玉琛．醇溶蛋白电泳在小麦种质资源遗传分析中的应用[J]．中国农业科学，1995，28(4)：25-32．(Zhang X Y, Yang X M, Dong Y S．Genetic analysis of wheat germplasm by acid polyacrylamide gel electrophoresis of gliadins[J]．Scientia Agricultura Sinica， 1995，28(4)：25-32．)

[4]李正玲，许为钢，张清珍，等．河南省地方小麦品种醇溶蛋白的遗传多样性分析[J]．麦类作物学报，2008，28（4）：582-587．(Li Z L,Xu W G,Zhang Q Z,et al．Genetic heterogeneity and diversity within and among Henan wheat landraces with the same name as revealed by gliadin composition[J]．Journal of Triticeae Crops， 2008，28（4）：582-587．)

[5]Draper S R ．ISTA variety committee report of the working group for biochemical tests for cultivar identification 1983-1986[J]．Seed Science and Technology， 1987，15：431-434．

[6]Zillman R R，Bushuk W．Wheat cultivar identification by gliadin electrophoregramsⅡ．Effects of environmental and experimental factors on the gliadin electrophore-grams[J]．Canadain Journal of Plant Science， 1979，59：281-286．

[7]张学勇，杨欣明，董玉琛．醇溶蛋白电泳在小麦种质资源遗传分析中的应用[J]．中国农业科学，1995，28（4）：25-32．(Zhang X Y,Yang X M,Dong Y S．Genetic analysis of wheat germplasm by acid polyacrylamide gel electrophoresis of gliadins[J]．Scientia Agricultura Sinica， 1995，28(4)：25-32．)

[8]范莉英，李德颖，李敏．应用种子蛋白电泳图谱对高羊茅品种进行鉴别与聚类研究[J]．草业学报， 1996，5（4）：5-10．(Fan L Y,Li D Y,Li M．Identification and cluster analysis of tall fescue (Festuca arundinacea Schreb. ) cultivars using SDS-PAGE diagram of seed protein [J]．Acta Prataculturae Sinica， 1996，5（4）：5-10．)

[9]Laemmli U K．Cleavage of structural proteins during the assembly of the head of bacteriophage T4 [J]. Nature，1970，227：680-685．

[10]Woyehick J H，Boundy J A，Dimler R J．Starch gel electrophoresis of wheat gluten proteins with concentric area[J]．Archives of Biochemistry and Biophysics， 1961，94（3）：477-482．

[11]Nei M．Mathem atical model for studying gentic variationin termsok restriction endouudease[J]． Proceedings of the National Academy of Sciences of the United States of America，1979，76：5269-5273．

[12]Mellish A，Coulman B，Ferdinandez Y．Genetic relationships among selected crested wheatgrass cultivars and species determined on the basis of AFLP markers[J]．Crop Science， 2002，42：1662-1668．

[13]杨瑞武，魏秀华，周永红，等．赖草属植物醇溶蛋白的遗传多态性[J]．云南植物研究，2004，26(1)：103-110．（Yang R W,Wei X H,Zhou Y H, et al．Genetic polymorphism of gliadin in leymus [J]．Acta Botanica Yunnanica 2004，26(1)：103-110．）

[14]马啸，周永红，余海清，等．野生垂穗披碱草种质的醇溶蛋白遗传多样性分析[J]．遗传，2006，28(6)：699-706．(Ma X, Zhou Y H, Yu H Q, et al．Genetic diversity of gliadin in wild germplasm of Elymus nutans?Griseb[J]．Hereditas， 2006，28(6)：699-706．)

[15]郎明林，卢少源，张荣之．中国北方冬麦区主栽品种醇溶蛋白组成的遗传演变分析[J]．作物学报，2001，27(6)：958-966．(Lang M L,Lu S Y,Zhang Y Z．Analysis of the genetic evolution of gliadin composition in the major wheat cultivars grown in north China[J]．Acta Agronomica Sinica， 2001，27(6)：958-966．)

[16]魏育明，郑有良，刘登才，等．四川小麦地方品种Gli-1、Gli-2和Glu-1位点的遗传多样性[J]．植物学报，2000，42(5)：496-501．(Wei Y M, Zheng Y L, Liu D C, et al．Genetic diversity of Gli-1, Gli-2 and Glu-1 alleles in Sichuan wheat landraces[J]．Journal of Integrative Plant Biology， 2000，42(5)：496-501．)

[17]杨瑞武，魏秀华，周永红，等．赖草属植物醇溶蛋白的遗传多态性[J]．云南植物研究，2004，26(1)：103-110．（Yang R W,Wei X H,Zhou Y H,et al．Genetic polymorphism of gliadin in Leymus [J]．Acta Botanica Yunnanica， 2004，26(1)：103-110．）

[18]兰秀锦，郑有良，刘登才，等．山羊草属植物醇溶蛋白的遗传多样性分析[J]．草业学报，2006，15(6)：93-100．(Lan X J, Zheng Y L, Liu D C, et al．Gliadin polymorphism of Aegilops species[J]．Acta Prataculturae Sinica，2006，15(6)：93-100．)

[19]马小军，汪小全，徐昭玺．人参不同栽培群体遗传关系的RAPD分析[J]．植物学报，2000，42（6）：587-590．(Ma X J,Wang X Q,Xu Z X．RAPD variation within and among populations of ginseng cultivars[J]．Journal of Integrative Plant Biology， 2000，42（6）：587-590．)

[20]刘果厚，贾宝丽．浑善达克沙地榆遗传多样性的研究[J]．干旱区资源与环境，2003，17（5）：123-128．(Liu G H,Jia B Li．The study of genetic diversity of sabulosa var.Ulmus pumila?[J]．Journal of Arid Land Resources and Environment，2003，17（5）：123-128．)

[21]孙坤，陈纹，马瑞君．子午岭中国沙棘亚居群的遗传多样性研究[J]．兰州大学学报， 2004，40（3）：72-75．(Sun K,Chen W,Ma R J．A study on the genetic diversity of subpopulations of Hippophae rhamnoides?ssp. sinensis?at Ziwuling, Gansu[J]．Journal of Lanzhou University ，2004，40（3）：72-75．)


Memo

Memo:
-
Last Update: 2014-09-14