|Table of Contents|

Optimation on Fermentation Conditions of a High-yield Nattokinase Mutant(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年04期
Page:
692-695
Research Field:
Publishing date:

Info

Title:
Optimation on Fermentation Conditions of a High-yield Nattokinase Mutant
Author(s):
LI Wen-liang; BIAN Ming-di; WANG Hai-bo
College of Quartermaster Technology,Department of Agronomy,Jilin University,Changchun 130062，Jilin，China
Keywords:
Natto; Nattokinase; Fibrin plate; Conditions optimization
PACS:
TQ464.8
DOI:
10.11861/j.issn.1000-9841.2010.04.0692
Abstract:
In order to obtain optimal conditions for nattokinase production，the influencing factors of carbon source（glucose，sucrose）,nitrogen source（beef extract，peptone，soy peptone，yeast extract，ammonia sulfate），C/N concentration and inorganic ions composition were investigated by fermentation culture and the activity of nattokinase was determined by fibrin plate method. Results showed that the best condition of enzyme production was 1.5% peptone，1.5% maltose，0.05% MgSO4，0.2%CaCl2，0.2%NaH2PO4 and 0.1%Na2HPO4,with the carbon and nitrogen concentration ratio of 1∶1.

References:

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Last Update: 2014-09-15