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Difference of β-carotene Bleaching of Lipoxygenase Extracts from Soybean with Various Seed Lipoxygenase Isoenzymes(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年04期
Page:
681-687
Research Field:
Publishing date:

Info

Title:
Difference of β-carotene Bleaching of Lipoxygenase Extracts from Soybean with Various Seed Lipoxygenase Isoenzymes
Author(s):
MA Hao; ZHANG Xue-dan; WANG Xian-sheng; HE Xiao-ling
Nanjing Agricultural University, Nanjing 210095，Jiangsu，China
Keywords:
Glycine max (L.) Merrill; Lipoxygenase isoenzymes; Lox extract; β-carotene; Bleaching
PACS:
TS201.2
DOI:
10.11861/j.issn.1000-9841.2010.04.0681
Abstract:
β-carotene bleaching activity of Lox extracts from soybeans containing various Lox isoenzyme combinations was investigated under different extraction and reaction conditions. Results indicated that the optimum extracting solution for Lox1, Lox2, Lox3, Lox1,2, and Lox1,2,3 was Tris-HCl buffer(pH 6.8), while for Lox1,3 and Lox2,3, was sodium phosphate buffer(pH 6.0). The reaction time needed for extracts to bleach β-carotene completely was in the order of Lox1,2,3, Lox3>Lox2,3, Lox1,3> Lox1,Lox2>Lox1,2. At their corresponding optimum reaction temperatures, the bleaching levels of extracts was in the order of Lox1,3>Lox1,2,3, Lox2,3>Lox3>Lox1,2, Lox1>Lox2, while at their corresponding optimum pH values in the order of Lox1,3, Lox2,3>Lox1,2,3>Lox1,2>Lox2>Lox3>Lox1. With the laying time prolonged, the bleaching activity of extracts tended to decrease. Lox3 was indispensable for higher efficiency of β-carotene bleaching. The extracts of the isoenzyme combinations containing Lox3 (Lox1,3, Lox2,3, and Lox1,2,3) were found to have stronger bleaching activity than those without Lox3 or of single isoenzyme.

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