Indentification of the Strain Snea49 and Evaluation for Nematicidal Activity Against Soybean Cyst Nematode(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2010年04期

Page:

663-665

Research Field:

Publishing date:

Info

Title:

Indentification of the Strain Snea49 and Evaluation for Nematicidal Activity Against Soybean Cyst Nematode

Author(s):

CHEN Jing-sheng¹; 2; CHEN Li-jie¹; LIU Da-wei¹; ZHU Xiao-feng¹; WANG Yuan-yuan¹; DUAN Yu-xi¹

1.Plant Nematology Laboratory, Shenyang Agricultural University, Shenyang 110161,Liaoning；

2.Daqing Branch, Heilongjiang Academy of Agricultural Sciences, Daqing 163316, Heilongjiang, China

Keywords:

Actinomycetes; Heterodera glycines; 16S rDNA; second stage juvenile （J2）

PACS:

S154.38

DOI:

10.11861/j.issn.1000-9841.2010.04.0663

Abstract:

Strain Snea49，was isolated by diluted solution from a soil sample collected in Liaoning province.The results based on characteristics of morphological，physiology and biochemistry and 16S rDNA sequence analysis of strain Snea49 showed that it was Streptomyces anulatus. Fermentation filtrates of Streptomyces anulatus at different concentrations were evaluated for nematicidal activity against soybean cyst nematode in the laboratory，bioassays included egg hatching and mortality of second stage juvenile.The hatching rate of cysts were suppressed by 82.60% at 10× diluted solution. The corrected mortality rates of juveniles were 89.66% when J2 were treated with the strain at 1× dilution for 24 hours. The difference between these treatments and water control were significant. Fermentation filtrates of the strains were effective in inhibiting H. glycines?hatch and killing J2.

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