|Table of Contents|

Screening of Superior Soybean Rhizobial Strains and Approach to Inoculation Methods for Region of HuangHuaiHai(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年04期
Page:
645-650
Research Field:
Publishing date:

Info

Title:
Screening of Superior Soybean Rhizobial Strains and Approach to Inoculation Methods for Region of HuangHuaiHai
Author(s):
LI Tao1; 2; GUAN Da-wei2; LI Jun2; CAO Feng-ming2 ; WEI Ge-hong1; FENG Rui-hua2
1.College of Life Sciences,Northwest A ＆ F University,Yangling 712100,Shaanxi;
2.Institute of Agricultural Resources and Agricultural Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 10081,China
Keywords:
Rhizobium; Soybean; Superior strain; Inoculation method
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.04.0645
Abstract:
The superior rhizobia strains matched well with the soybean (Glycine max) cultivar Fudou 9765 were screened from 22 strains of rhizobia,which were isolated from 11 regions in 3 provinces adopting vermiculite pot and soil pot.Subsequently,three inoculation methods were compared in field experiments.By vermiculite pot experiment,6 strains were selected out according the number of nodules,dry weights and total N contents,and in the subsequent soil pot experiment the best two strains of Bradyrhizobium japonicum 4302 and Sinorhizobium fredii 4822 were selected by their performance of nitrogen fixing and competitive nodulation ability from the 6 strains.The field experiment about three inoculation methods of seed dressing,spraying and below the soybean seeds were conducted,and the method of seed dressing showed the best results than the other two treatments with higher plant dry-weight,total N content of plant,nodulation occupancy and seed yield.It indicated that the inoculation method of seed dressing was suitable for rhizobium application in Huanghuaihai region.

References:

[1]李阜棣.共生固氮与根瘤菌在持续农业和环境保护中的意义[M].武汉:武汉出版社,1998.(Li F D.The significance of symbiotic nitrogen fixation with rhizobia in the sustainable agriculture and environmental protection[M].Wuhan:Wuhan Press,1998.)

[2]Marta A,Dulce N R-N,Francisco J T.Soybean inoculation:Dose,N fertilizer supplementation and rhizobia persistence in soil[J].Field Crops Research,2009,113:352-356.

[3]Lilian M,Fabio L M,Beatriz G D,et al.Selection of bean (Phaseolusvulgaris L.) rhizobial strains for the Brazilian Cerrados[J].Field Crops Research,2002,73:121-132.

[4]Alves B J R,Boddey R M,Urquiaga S.The success of BNF in soybean in Brazil[J]. Plant and Soil,2003,252:1-9.

[5]Bogino P,Banchio E,Bonfiglio C,et al.Competitiveness of a Bradyrhizobiumsp.strain in soils containing indigenous rhizobia[J].Current Microbiology,2008,56:66-72.

[6]王彩洁,徐冉,张礼凤,等.黄淮海地区大豆推广品种的状况与育种发展趋势[J].山东农业科学，2006(5):14-16.(Wang C J,Xu R,Zhang L F,et al.The situation of promotion soybean variety and trend of breeding development in region of Huang-Huai[J].Shandong Agricultural Sciences,2006(5):14-16.)

[7]关大伟,李俊,曹凤明.黄淮大豆产区土壤养分状况与根瘤菌应用现状及对策[J].大豆科技,2009(2):9-11.(Guan D W,Li J,Cao F M.The status of soil nutrients and the current situation and countermeasures of rhizobium application[J].Soybean Technology,2009(2):9-11.)

[8]江木兰,张学江,徐巧珍,等.大豆-根瘤菌的固氮作用[J].中国油料作物学报,2003,25(1):50-53,58.(Jiang M L,Zhang X J,Xu Q Z,et al.Nodulation and nitrogen-fixation in soybean-rhizobium[J].Chinese Journal of Oil Crop Sciences,2003,25(1):50-53,58.)

[9]曾昭海.紫花首蓓高效根瘤菌筛选及田间鉴定方法研究[D].北京：中国农业大学,2003:95.(Zeng Z H.Study on the screening of high-efficient Sinorhizobium melilotifor Medicago sativaL.cultivars and the identification in field[D].Beijing:China Agricultural University,2003:95.)

[10]窦新田,周俊初.大豆基因工程根瘤菌田间结瘤和共生固氮效应[J].生物技术,1991,1(3):26-29.(Dou X T,Zhou J C.The effect of symbiotic nitrogen fixation of gene engineering rhizobium strains in soybean in the field[J].Biotechnology,1991,1(3):26-29.)

[11]戴小密,刘彦杰,叶小梅,等.接种大豆根瘤菌( Sinorhizobium fredii)遗传工程菌株LMG101对大豆的增产效应[J] .中国农业科学,2003,36 (1) :66-70.(Dai X M,Liu Y J,Ye X M,et al.Soybean yield response to inoculation with genetically engineered strain LMG101 of Sinorhizobium fredii[J].Scientia Agricultura Sinica,2003,36 (1):66-70.)

[12]陈强,张小平,陈文新,等.从豆科植物的根瘤中直接提取根瘤菌DNA的方法[J].微生物学通报,2002,29(6):63-67.(Chen Q,Zhang X P,Chen W X,et al.Isolation of DNA from the root nodule of legume plant[J].Microbiology,2002,29(6):63-67.)

[13]Jia R Z,Tian C F,Man C X,et al.Screening of high effective alfalfa rhizobial with a comprehensive protocol[J].Annals of Microbiology,2008,58(4):1-9.

[14]缪礼鸿,周俊初.根瘤菌竞争结瘤的研究进展[J].华中农业大学学报,2003,22(1):84-87.(Miao L H,Zhou J C,Advance on the study of the nodulation competition of rhizobia[J].Journal of Huazhong Agricultural University 2003,22(1):84-87.)

[15]卢林纲.黑龙江省大豆根瘤菌复合颗粒肥的研制及其应用技术研究[D].北京：中国农业大学,2005:21-27.(Lu L G.Developing Bradyrhizobium japonicummixed pellet fertilizer and its application in Heilongjiang Province[D].Beijing:China Agricultural University,2005:21-27.)

[16]Maria J A,Silvina L.Strain selection for improvement of Bradyrhizobium japonicum competitiveness for nodulation of soybean[J].FEMS Microbiology Letters,2008,282:115-123.

[17]沈辉,周俊初,吴魁斌,等.大豆根瘤菌基因工程菌株NH32田间竞争结瘤能力初报[J]．微生物学研究与应用,1993(1):15-18.(Shen H,Zhou J C,Wu K B,et al.The preliminary report for competitive nodulation ability in field of the genetically engineered strain of soybean RhizobiumNH32[J].Microbiology Research and Application,1993(1):15-18.)

[18]张学江,姚瑞林,江木兰.花生根瘤菌数量效应与种床接种效果[J].中国油料,1991(2):56-59.(Zhang X J,Yao R L,Jiang M L.Responses of peanut to rhizobium numbers and seed bed inoculation[J].China Oil,1991(2):56-59.)

[19]唐颖,卢林纲,隋文志,等.根瘤菌不同接种方式对大豆根瘤分布及产量的影响[J].现代化农业,2002(4):13-14.(Tang Y,Lu L G,Sui W Z,et al.Effection of yield and distribution of nodules in response to rhizobium inoculation methods[J].Modernization Agriculture,2002(4):13-14.)

[20]Nadia N,Weaver R W.Number of Rhizobia and delayed inoculation influence nodulation of clovers[J].Plant and Soil,1994,158:135-139.


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Last Update: 2014-09-14