|Table of Contents|

Comparison and Optimization of the Agrobacterium-mediated Transformation of Soybean by Using Cotyledonary Node and Hypocotyl Explants(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年04期
Page:
590-593,597
Research Field:
Publishing date:

Info

Title:
Comparison and Optimization of the Agrobacterium-mediated Transformation of Soybean by Using Cotyledonary Node and Hypocotyl Explants
Author(s):
DUAN Ying-yingZHAO LinCHEN Li-miaoLI Wen-bin
Soybean Research Institute,Northeast Agricultural University,Key Laboratory of National Educational Department,Harbin 150030,Heilongjiang,China
Keywords:
SoybeanCotyledonary node transformationHypocotyl transformation
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.04.0590
Abstract:
Soybean cotyledonary node and hypocotyl transformation methods were compared and optimized using three soybean genotypes, Dongnong 50, Dongnong 42 and Heinong 44.The results showed that different transformation methods required different soybean varieties. Dongnong 50 was suitable to cotyledonary node transformation method, while Heinong 44 was suitable to hypocotyl transformation method. In the cotyledonary node transformation system, the screening concentration of Kanamycin was 100 mg·L-1, while in the hypocotyl transformation system was 75 mg·L-1,the hypocotyl transformation system was more sensitive than the cotyledonary node transformation system to kanamycin. The optimal concentration of acetosyringone was both 200 mol·L-1,the optimal co-culture time was both 3 d in the cotyledonary node and hypocotyl transformation systems. In the cotyledonary node transformation system, conversion rate was the highest when the seedling age was 5 d, while in the hypocotyl transformation system, conversion rate was higher in 4-6 d, in which the highest was 6 d.

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