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Reveal the Evolutionary Relationship of Soybean Germplasm by Comparing 18S rRNA Gene Sequences(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年04期
Page:
586-589
Research Field:
Publishing date:

Info

Title:
Reveal the Evolutionary Relationship of Soybean Germplasm by Comparing 18S rRNA Gene Sequences
Author(s):
LIANG Jiang1;  CHEN Yuan1;  TANG Fu-yue1;  WEI Qing-yuan1;  YUAN Qing-hua1;  2
1.Guangxi Maize Research Institute, Nanning 530227;
2.Guangxi Crop Genetic Improvement and Biotechnology Lab,Nanning 530007, Guangxi，China
Keywords:
Glycine soja; Glycine max; Arabidopsis thaliana; 18S rRNA gene
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.04.0586
Abstract:
The evolution relationships between Glycine soja and Glycine max were studied by comparing the partial gene sequences of their 18S rRNA in this article. The results were as follows:1 000 bp 18S rRNA genes of 3 wild soybeans and 3 cultivated ones were amplified using primers which were designed according to Arabidopsis thaliana 18S rRNA genes, the homology of 18S rRNA was 99% among the wild soybeans, while was from 97% ~ 98% among cultivated soybeans. By studying the evolutionary relationships of different legume crops with 18S rRNA sequence, we found that soybean was near the root of phylogenetic tree, which suggested that soybean was much more close to the phylogenetically primitive position compared to other legume crops. The results above suggestted that soybean cultivars should become more diversified in the process of evolution, and the fact that soybean varieties had narrow genetic background may be due to human intervention in the process of selecting. In conclusion, 18S rRNA gene sequence could reflect the evolutionary relationships between different soybean varieties, which will provide theoretical basis for the utilization of soybean germplasm resources.

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Last Update: 2014-09-14