|Table of Contents|

Introduction of Anti-pest Gene (cry V) to Soybean via Pollen-Tube Pathway(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年04期
Page:
565-568,574
Research Field:
Publishing date:

Info

Title:
Introduction of Anti-pest Gene (cry V) to Soybean via Pollen-Tube Pathway
Author(s):
WU Xiao-xia1;  LIU Wei-ting2;  LIU Qi3;  LI Jing1;  MA Yong4;  LI Wen-bin1
1.Agricultural College of Northeast Agricultural University,Chinese Education Ministry’s and Provincial Key Laboratory of Soybean Biology, Harbin 150030；
2.Plant Virus-free Nursery Stock Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086;
3.Horticulture Branch of Heilongjiang Academy of Agricultural Sciences, Harbin 150086;
Keywords:
Soybean; Anti-pest gene（cry V）; Pollen-tube pathway
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.04.0565
Abstract:
Anti-pest gene (cry V) was introduced to soybean via pollen-tube pathway during blossom period. 1211 flowers out of 2760 ones survived 3 days after the operation, the survival rate and podding rate was 43.88%and 33.6%, respectively. The harvested seeds were planted in greenhouse and 5 plants with positive result were obtained after Km screening, PCR identification, southern-blot testing and RT-PCR testing. Results suggestted that the anti-pest gene (cry V) were successfully introduced into those 5 plants and the transformation rate was 1.8‰.

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Last Update: 2014-09-14