|Table of Contents|

Cloning and Sequence Analysis of HC-Pro Gene of Soybean Mosaic Virus(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年04期
Page:
549-554
Research Field:
Publishing date:

Info

Title:
Cloning and Sequence Analysis of HC-Pro Gene of Soybean Mosaic Virus
Author(s):
LIU Ning1; 2;  LIU Ruo-miao1;  MA Ying1;  WANG Da-gang1;  ZHI Hai-jian1
1.National Center for Soybean Improvement of Nanjing Agricultural University,National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095,Jiangsu;
2.Yantai Entry-Exit Inspection and Quarantine Bureau, Yantai 264000,Shandong,China
Keywords:
Soybean mosaic virus;  HC-Pro gene;  Gene cloning;  Sequence analysis
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.04.0549
Abstract:
This study aims to clone and sequence full-length cDNA of HC-Pro gene of six SMV isolates using RT-PCR, to observe the reaction of six SMV isolates to ten hosts, and to discuss the relationships between similarity of the gene sequence and virus virulence, effect of cross-protection. The results showed that full-length cDNA of HC-Pro gene had 1371 nucleotides encoding 457 amino acids. The protein HC-Pro had functional domains such as the CCC motif related to virus movement and the PTK motif related to spread by aphids. Homologies of six SMV isolates nucleotides were 89.2%~99.9% and amino acids were 95.4%~99.8%. There was one different nucleotide in sequence of HC-Pro gene between TS216 and 4434. No certain relationship was found between homology of HC-Pro gene of six SMV isolates and their virulence. While, homology of SMV isolates determined effects of cross-protection to a certain extent.

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Last Update: 2014-09-14