|Table of Contents|

Optimization for ISSR-PCR Reaction System of Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年03期
Page:
510-513
Research Field:
Publishing date:

Info

Title:
Optimization for ISSR-PCR Reaction System of Soybean
Author(s):
HE Hai-yan; SHA Wei; ZHANG Yan-fu
College of Life Science and Engineering, Qiqihar University, Qiqihar 161006,Heilongjiang，China
Keywords:
Soybeen; ISSR-PCR; System optimization
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.03.0510
Abstract:
Orthogonal design was adopted to optimize ISSR-PCR amplification system on soybean in five factors(Mg2+,dNTPs,primer,DNA template and Taq DNA polymerase)at four levels respectively, with 25 soybean germplasm from Heilongjiang Province as material. The most suitable ISSR-PCR system for soybean was established, namely 25 μL reaction system containing 1.85 mmol·L-1Mg2+,1.2mmol·L-1dNTPs,60 ng DNA template, 1.2μmol·L-1primer, and 0.7 U Taq DNA polymerase. Underthe optimized reaction conditions, 25 soybean were easily amplified with primer 855. The result provided a standardizing program for the analysis of interspecies genetic diversity of soybean of Heilongjiang Province.

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Last Update: 2014-09-14