Optimization of Soybean Transformation System and Transferring Dof 4 Gene into Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2010年03期

Page:

398-402

Research Field:

Publishing date:

Info

Title:

Optimization of Soybean Transformation System and Transferring Dof 4 Gene into Soybean

Author(s):

DU Sheng-wei¹;  2; LIU Ye-li²; YAO Bing-chen¹;  2; BAI Chen²; MIAO Xing-fen¹; LIU Chun-yan²; CHEN Qing-shan²; HU Guo-hua¹;  2

1. College of Agriculture, Northeast Agricultural University, Harbin,150030;?

2.Crop Research and Breeding Center of Land-Reclamation, Harbin 150090,Heilongjiang,China

Keywords:

Soybean; Cotyledonary node; Dof 4; Agrobacterium-mediated transformation

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2010.03.0398

Abstract:

In this paper, some major factors, which played important role in improving the transformation efficiency of soybean, such as the method of sterilizing the seeds, plant hormones, and antibiotics, were optimized. The Dof 4 was transferred into the soybean cultivar Suinong 14 by Agrobacterium-mediated cotyledonary node transformation. The result showed that, the chlorine suffocating was chosen to seeds sterilize, which did not harm to the seeds and have a higher differentiation rate for multiple shoot. However, duration of Agrobacterium infection and co-culture influenced the contamination and the state of multiple shoot. As for shoot induction, a higher regenerated rate, better callus induction rate, and lower deformity rate were obtained with 1.6 m·L^－16-BA and 0.1m·mL^-1IBA in this experiment. And the optimum combination of antibiotic and concentration in medium was 200 m·mL^-1Cefotaxime Sodium and 250 mg·mL^-1Carbenicillin.

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