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Cloning and Identification of the Seedcoat Specific Promoter from Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年03期
Page:
390-393
Research Field:
Publishing date:

Info

Title:
Cloning and Identification of the Seedcoat Specific Promoter from Soybean
Author(s):
WEI Wen-wen;  WANG Pi-wu;  FU Yong-ping;  Zhang Zhuo
College of Agriculture, Jilin Agricultural University, Changchun 130118, Jilin, China
Keywords:
Soybean; Seedcoat specific promoter; Functional Identification; GUS staining
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.03.0390
Abstract:
The promoter region of Seedcoat-specific gene was isolated from the genomic DNA of soybean Jilin 17 by PCR method, and obtained the promoter fragment SCSP, the length of the clone sequence is 1268bp. Sequence analysis indicated that this fragment had 97% homology compared with the reported promoters. The cloned promoter was fused to the GUS reporter gene to construct plant expression vector pSCSP-GUS,which was transferred into tobacco(Nicotiana tabacum)NC89 by Agrobacterium tumefaciens-mediated method and severa1transformed plants were obtained.GUS activity assays of different soybean organs indicated that expression of GUS was active only in seedcoat,which suggests the SCSP gene is seedcoat-specific promoter.

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