|Table of Contents|

Cloning and Mapping of Telomere Associated Sequence in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年03期
Page:
380-384
Research Field:
Publishing date:

Info

Title:
Cloning and Mapping of Telomere Associated Sequence in Soybean
Author(s):
WU Wei1;  2;  LI Hong-jie2;  WANG Xiao-ming2;  ZHU Zhen-dong2;  DONGFANG-Yang1
1. Academic center of Life Science, Hebei Normal University of Science and Technology, Changli 066600, Hebei；
2. Institute of Crop Science, Chinese Academy of Agricultural Sciences,National Key Facility of Crop Gene Resource and Genetic Improvement, Beijing 100081,China
Keywords:
Soybean cultivar; Telomere associated sequence; Telomeric repeat motif; Clone; Mapping
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.03.0380
Abstract:
Using the Arabidopsis-type telomeric repeat (TTTAGGG)3 as a primer, a 574 bp TAS was amplified and cloned from three soybean (Glycine max) cultivars. Sequence analysis indicated that the similarities of the TASs from different cultivars in this study and other TASs from GenBank ranged from 92% to 99%. A similarity of 79% was detected between the TAS obtained and the interval sequence of TR-TAS from Silene latifolia. Low similarities ranging from 16% to 35% were observed between the soybean TAS and other plants such as barley (Hordeum vulgare) and corn (Zea mays). In this TAS fragment, there were 14 copies of Arabidopsis-type telomeric repeat (TTTAGGG/CCCTAAA) and 30 copies of mutation motifs with deletion, substitution and inversion. The 25 bp conserved tandem repeat in this TAS fragment occurred in 13 copies and the content of adenine and thymine (A+T) was greater than 60%, showing the characterization of satellite DNA. This TAS fragment was mapped to the proximal end of soybean chromosome 3.

References:

[1]Richards E J, Ausubel F M. Isolation of a higher eukaryotic telomere from Arabidopsis thaliana [J]. Cell, 1988, 53: 127-136.

[2]Ganal M W, Lapitan N L, Tanksley S D. Macrostructure of the tomato telomeres [J]. Plant Cell, 1991, 3: 87-94.

[3]Werner J E, Kota R S, Gill B S, et al. Distribution of telomeric repeats and their role in healing of broken chromosome ends in wheat [J]. Genome, 1992, 34: 844-848.

[4]Burr B, Burr F A, Matz E C, et al. Pinning down loose ends: mapping telomeres and factors affecting their length [J]. Plant Cell, 1992, 4: 953-960.

[5]Wu T Y, Wang Y X, Wu R. Transcribed repetitive DNA sequences in telomeric regions of rice (Oryza sativa) [J]. Plant Molecular Biology, 1994, 26: 363-375.

[6]Fajkus J, Kovarik A, Kralovics R, et al. Organization of telomeric and subtelomeric chromatin in the higher plant Nicotiana tabacum [J]. Molecular and General Genetics, 1995, 247:633-638.

[7]张德水, 张志永, 陈受宜. 大豆染色体端粒DNA的Southern及荧光原位杂交(FISH)分析[J]. 高技术通讯, 1998, 12: 7-10. (Zhang D S, Zhang Z Y, Chen S Y. Southern blotting and FISH analysis of telomeric DNAs in soybean [J]. High Technology Letters, 1998, 12: 7-10.)

[8]Zakian V A. Telomeres: beginning to understand the end [J]. Science, 1995, 270: 1601-1607.

[9]Mao L, Devos K M, Zhu L, et al. Cloning and genetic mapping of wheat-associated sequences [J]. Molecular and General Genetics, 1997, 254: 584-591.

[10]巩学千, 龚继明, 刘峰, 等. 筛选和定位含水稻端粒相关序列的BAC克隆[J]. 中国科学(C 辑), 1998, 28: 437-443. (Gong X Q, Gong J M, Liu F, et al. Isolation and mapping of bacterial artificial chromosome (BAC) clone containing telomere-associated sequence [J]. Science in China (series C), 1998, 28: 437-443.)

[11]Singh R J, Hymowitz T. The genomic relationship between Glycine max(L.)Merr and G.soja?Sieb and Zucc as revealed by pachytene chromosome analysis [J]. Theoretical and Applied Genetics, 1988, 76: 705-711.

[12]张德水. 大豆基因组分子连锁图的构建及染色体端粒相关序列的克隆与特性分析[D]. 北京：中国科学院遗传与发育生物学研究所, 1997: 60-72. (Zhang D S. Construction of a molecular linkage map and cloning and characterization of telomere associated sequences in soybean[D]Beijing: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 1997: 60-72.)

[13]王珍, 方宣钧. 植物DNA分离[J]. 分子植物育种, 2003,l(2): 281-288. (Wang Z, Fang X J. Plant DNA isolation [J]. Molecular Plant Breeding, 2003,l(2): 281-288.)

[14]Sykorová E, Lim K Y, Kunická Z, et al. Telomere variability in the monocotyledonous plant order Asparagales [J]. Proceedings of Royal Society B Biological Science, 2003, 270: 1893-1904.

[15]Allshire R C, Dempster M, Hastie N D. Human telomeres contain at least three types of G-rich repeat distributed non-randomly [J]. Nucleic Acids Research, 1989, 17: 4611-4627.

[16]巩学千, 洪德军, 陈受宜, 等. 水稻端粒相关序列的克隆及鉴定[J]. 中国科学(C辑), 1998, 28: 324-332. (Gong X Q, Hong D J, Chen S Y, et al. The cloning and characterization of rice telomere associated sequence [J]. Science in China (series C), 1998, 28: 324-332.)

[17]Riha K, Fajkus J, Siroky J, et al.Developmental control of telomere lengths and telomerase activity in plants [J]. Plant Cell, 1998, 10: 1691-1698.

[18]Sykorová E, Cartagena J, Horáková M, et al. Characterization of telomere-subtelomere junctions in Silene latifolia [J]. Molecular Genet Genomics, 2003, 269: 13-20.

[19]Cohn M, Edstrom J E. Chromosome ends in Chironomus pallidivittatus contain different subfamilies of telomere-associated sequences [J]. Chromosoma, 1992, 101: 634-640.

[20]Kato A, Lamb J C, Birchler J A. Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize [J]. Proceedings of the National Academy of Sciences USA, 2004, 101: 13554-13559.


Memo

Memo:
-
Last Update: 2014-09-13