|Table of Contents|

Evaluation on the Competitiveness of Strains of Soybean Rhizobia Marking with gfp and rfp Genes(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年03期
Page:
366-369
Research Field:
Publishing date:

Info

Title:
Evaluation on the Competitiveness of Strains of Soybean Rhizobia Marking with gfp and rfp Genes
Author(s):
XIAO Wen-li GUAN Da-wei LI Jun CAO Feng-ming CHEN Hui-jun
Institute of Agricultural Resources and Agricultural Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 10081, China
Keywords:
Fluorescent protein gene Rhizobium Competitive nodulation
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.03.0366
Abstract:
The green-fluorescent protein gene (gfp) or red-fluorescent protein gene (rfp) was transferred into Sinorhizobium fredii SR25a, S.fredii USDA205, Bradyrhizobium japonicum4222, B.japonicum 4230 and B.japonicum 4534 by double-parent hybridizing method respectively to obtain 6 marked strains. The genetic stability of the foreign genes were detected and their effects on the strains nodulation ability were evaluated. Nine pairs of strains from testing strains with different fluorescent protein gene were inoculated on the soybean cultivar Zhonghuang 13 for competitive nodulation experiment. The results showed that the foreign genes could transfer stably, and they had no impact on the symbiotic nitrogen fixation activity of strains. The competitive nodulation ability of B.japonicum was higher than S.fredii. Among the B.japonicum strains, nodule occupancy of B.japonicum 4534 was the highest and its nodule occupancy was 35% and 90% over B.japonicum 4230 and B.japonicum 4222, respectively. Marking with fluorescent protein gene provides one way to evaluate rapidly the competitive nodulation ability of rhizobial strains.

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Last Update: 2014-09-13