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Comparative Studies of RNA Editing of atp6 Gene between Cytoplasmic Male Sterile Line and Its Maintainer Line in Soybean (Glycine max L. Merr.)(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年03期
Page:
361-365
Research Field:
Publishing date:

Info

Title:
Comparative Studies of RNA Editing of atp6 Gene between Cytoplasmic Male Sterile Line and Its Maintainer Line in Soybean (Glycine max L. Merr.)
Author(s):
HAN Li-tao;  YANG Shou-ping;  YU De-yue;  GAI Jun-yi
Soybean Research Institute, Nanjing Agricultural University, National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, Jiangsu, China
Keywords:
Soybean;  Cytoplasmic male sterility(CMS);  Atp6 gene;  RNA editing
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.03.0361
Abstract:
The atp6 gene is an important mitochondrial functional gene. The studies on some plant species showed that RNA editing of atp6 gene was related to the cytoplasmic male sterility. In this paper, the comparative studies of RNA editing of atp6 gene between the cytoplasmic male sterile line NJCMS1A and its maintainer line NJCMS1B in soybean (Glycine max L. Merr.) were conducted. The results showed that two editing sites were found on the conservative region of the atp6 gene of NJCMS1A and NJCMS1B, but the two editing sites of the atp6 gene of NJCMS1A were different from those of NJCMS1B, and led to the different changes of amino acid. The results of the mtDNA sequence comparision showed that one base difference was found in the conservative region of atp6-3 gene between NJCMS1A and NJCMS1B. In addition, the obvious difference was found on the expression of atp6-1, atp6-2and atp6-3 between NJCMS1A and NJCMS1B.

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