|Table of Contents|

Adsorption of Nattokinase by Soybean Microspheres(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年02期
Page:
306-309.314
Research Field:
Publishing date:

Info

Title:
Adsorption of Nattokinase by Soybean Microspheres
Author(s):
MA Yue-hua12 DONG Chao2YANG Ming12 SHI Yan-mao2
1.College of Chemistry and Chemical Engineering, Hebei University of Technology, Tianjin 300100;?
2. Hebei Institute of Biology, Shijiazhuang 050081,Hebei,China
Keywords:
Soybean microspheres Nattokinase Adsorption equilibrium Adsorption dynamics
PACS:
Q814
DOI:
10.11861/j.issn.1000-9841.2010.02.0306
Abstract:
The affinity adsorption of Nattokinase by soybean microspheres was characterized and the static adsorption dynamics, adsorption isotherms and some adsorption conditions were investigated in the present work. The results revealed that the adsorption isotherm was accorded with Langmuir adsorption isotherm and the adsorption dynamics was coincident with diffusion equation. The best buffer was 0.01 mol·L-1PBS with pH of 6.0.Under this condition,the maximum adsorption capacity was 6 351.58 IU·g-1. The purification fold and recovery of Nattokinase activity reached 30.23 and 81.3%,respectively. It may be presumed that the soybean protein contains the ligand which absorb the nattokinase specifically.

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Last Update: 2014-09-13