|Table of Contents|

Purity Identification of “Meiqiao” Soybean (Glycine max) by SSR Markers(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年02期
Page:
210-214
Research Field:
Publishing date:

Info

Title:
Purity Identification of “Meiqiao” Soybean (Glycine max) by SSR Markers
Author(s):
SHENG Xin-ying1 ZHAN Shao-hua2 FAN Hong-hong1 CAI Yong-ping1 LIN Yi1
1.School of Life Science, Anhui Agricultural University, Hefei 230036;
2.Chemistry and Biology Department, West Anhui University, Liu’an 237012,Anhui,China
Keywords:
SSR Markers Soybean DNA extraction Germplasm Purity
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.02.0210
Abstract:
“Meiqiao”is a vegetable soybean with good quality in Huaibei Anhui Province, as result of continuous cultivation, the purity and yield reduced seriously. In this article, 92 plants were selected to identify the purity using SSR molecular markers so that it could provide theoretical guide for purification and rejuvenation of “Meiqiao” Soybean. The results showed that the high quality soybean genomic DNA was extracted by improved CTAB method, under optimization of SSR-PCR reaction system, eight SSR primers were selected and 10.6 bands were obtained from each one, the rate of polymorphic bands was70.9%, the purity of “Meiqiao” Soybean germplasm was 38.04%.

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Last Update: 2014-09-13