|Table of Contents|

Isolation and Analysis of Soybean Kunitz Trypsin Inhibitor Gene from Glycine tomentella(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年02期
Page:
191-194
Research Field:
Publishing date:

Info

Title:
Isolation and Analysis of Soybean Kunitz Trypsin Inhibitor Gene from Glycine tomentella
Author(s):
ZHAO Hong-kun LI Qi-yun WANG Yu-min LI Yu-qiu ZHUANG Bing-chang DONG Ying-shan
Biotechnology Research Center, Jinlin Academy of Agricultural Sciences, Changchun 130033,Jilin,China
Keywords:
Glycine tomentella Soybean Kunitz trypsin inhibitor Prokaryotic expression
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.02.0191
Abstract:
A single soybean Kunitz trypsin inhibitor gene,KTiPW54, was isolated from immature cotyledons of perennial wild soybean (G. tomentella) with high aphids resistance by RT-PCR. Sequencing analyses revealed a cDNA sequence of 654 bp long and encoded 218 amino acids. The deduced amino acid sequence of KTiPW54 exhibited high similarities to those of soybean Kunitz trypsin inhibitor. The coding region was fused to the intein of pWIN1 and over-expressed in E.coli BL21(DE3) induced by IPTG.

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