|Table of Contents|

Cytological and Genetical Characterization of A Nuclear Male-sterille Soybean Mutant NJS-1H(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年02期
Page:
181-185
Research Field:
Publishing date:

Info

Title:
Cytological and Genetical Characterization of A Nuclear Male-sterille Soybean Mutant NJS-1H
Author(s):
LI Shu-guang; ZHAO Tuan-Jie; GAI Jun-Yi
Soybean Research Institute of Nanjing Agricultural University, National Center for Soybean Improvement; National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, Jiangsu, China
Keywords:
Soybean;  Nuclear male sterility;  Abnormal synapsis;  Inheritance
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.02.0181
Abstract:
A soybean nuclear male-sterile mutant NJS-1H was obtained from NN86-4 induced with NaN3. A series of irregular phenomena were observed during its microsporogenesis based on normal squash and acetocarmine dyeing. The meiosis of pollen mother cells of its male sterile plants was highly abnormal in chromosome synapsis at prophase I, followed with appearing univalent, chromosome lagging, unequal and asynchrony distribution, etc. during the meiotic process. At tetrad stage, various malformed polyad and a large number of micronuclei appeared. Afterwards at uninuclear microspore stage, the nuclei degenerated with cytoplasm becoming washy and microspores shrunk and then the mature pollens appeared empty without cell inclusion and therefore were sterile. The abnormal phenomena occurred almost at all stages from initial of meiosis to pollen maturity with its major period during meiosis since the disorder of chromosome synapsis causing pollen degeneration. The results from parallel cross tests indicated that the NJS-1H male-sterile plant is also partially female-sterile. From the male fertility segregation analyses of progenies derived from male-fertile individuals obtained in fertility-segregation families, it was found that the male sterility of NJS-1H was controlled by a pair of recessive nuclear genes.

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