Ultrasonic-assisted Extraction of Total Lignans and Its Content Analysis inSoybeans(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2010年01期

Page:

168-170,173

Research Field:

Publishing date:

Info

Title:

Ultrasonic-assisted Extraction of Total Lignans and Its Content Analysis inSoybeans

Author(s):

CHEN Yun¹; SHI Zhan-wang²; HUANG Xiao-min¹

1.Bioscience Department of Shaoguan University，Shaoguan 512005，Guangdong;

2.Chemical and Ecological Engineering College of Guangxi Ethnic University，Nanning 530006，Guangxi，China

Keywords:

Soybean; Total lignans; Ultrasonic-assisted extraction; UV-Vis

PACS:

TS214.2

DOI:

10.11861/j.issn.1000-9841.2010.01.0168

Abstract:

Total lignans in several soybean samples were detected by UV-Vis with the ultrasonic-assisted extraction in this paper.The result showed that by twice extraction it can get ideal effect with ethanol as extracting reagent in the material/reagent ratio of 1/8~1/12.The relationship between standard substance concentration within 0~0 18 m·mL^-1 and absorbance remained better linear.The regression equation was A=6.081c+0.0445,with the correlation coefficient r=0.9998，the detection limit was 8.3×10-5 m·mL^-1with the average recovery of 94.4% and the RSD of 1.2%.Results suggest the method is convenient and practical to detect lignans from soybean.

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