|Table of Contents|

Separation and Purification of Soybean Isoflavone in Semen Sojae Praeparatum by ADS-7 Macroporous Adsorptive Resins(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年01期
Page:
164-167
Research Field:
Publishing date:

Info

Title:
Separation and Purification of Soybean Isoflavone in Semen Sojae Praeparatum by ADS-7 Macroporous Adsorptive Resins
Author(s):
NIU Li-ying; CAI Guang-hua; WANG Xin-guo
College of Traditional Chinese Medicine，Hebei Medical University，Hebei Key Lab of Laboratory Animal Science，Shijiazhuang 050091，Hebei，China
Keywords:
Semen Sojea Praeparatum; Soybean Isoflavone; Macroporous Adsorptive Resins; Adsorption; Separation
PACS:
R284.2
DOI:
10.11861/j.issn.1000-9841.2010.01.0164
Abstract:
Use ultraviolet spectrophotometry to detect the content of soybean isoflavone，with the content of genistein as the index，to investigate the ADS-7 macroporous adsorptive resins for separation and purification of soybean isoflavone in the Semen Sojea Praeparatum. The optimized technological parameters of the ADS-7 macroporous adsorptive resins were as following：loading sample density 0.225 ·mL-1，pH 4.5，the maximum sample volume 150 mL，4 B·h-1 flow rate to adsorption and the volume of 70%(v/v)ethanol as 8 BV with desorption-flow 4 B·h-1. With these conditions，more than 40% of soybean isoflavone could be obtained from primary adsorption and purification，and the ADS-7 macroporous adsorptive resins can be used twice.

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Last Update: 2014-09-13