|Table of Contents|

Protoplast Formation of Antagonistic Rhizobium(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年01期
Page:
92-96
Research Field:
Publishing date:

Info

Title:
Protoplast Formation of Antagonistic Rhizobium
Author(s):
WANG Yuan-yuan1; 2; DUAN Yu-xi 2; CHEN Li-jie2; ZHU Xiao-feng2
1.Department of Biological Science and Technology，Shenyang Agricultural University，Shenyang 110161；
2.Department of Plant Protection，Shenyang Agricultural University，Shenyang 110161，Liaoning，China
Keywords:
Rhizobium; Protoplast; Formation; Affecting factors
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.01.0092
Abstract:
Several factors affecting the protoplasts formation of Sinorhizobium.Fredii with antagonistic to SCN and root rot of soybean were studied.The growth curve was determined and the optimum cell-age for protoplasts formation was in the logarithmic growth mid-phase，the maximum yield of protoplast was accelerated when pretreated by 0.5% penicillium in the culture medium，the optimum enzyme condition was digesting at 35℃ with 1.5 mg?mL-1lysozyme，the process of protoplasts release was observed using microscope.The foundation for genetic breeding of S.Fredii has been laid by using the protoplasts.

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Last Update: 2014-09-13