Electronic Mapping and Structure Analysis of Key Enzyme Genes on Aspartic Acid Metabolic Pathway in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2010年01期

Page:

22-27

Research Field:

Publishing date:

Info

Title:

Electronic Mapping and Structure Analysis of Key Enzyme Genes on Aspartic Acid Metabolic Pathway in Soybean

Author(s):

YU Yan ¹; 2; JIANG Wei¹; 2; TANG Jing-xian³; LIU Chun-yan¹; 2; CHEN Qing-shan¹; 2; HU Guo-hua¹; 4

1.Agriculture College，Northeast Agricultural University，Harbin 150030，Heilongjiang；

2.The Crop Research and Breeding Center of Land-Reclamation，Harbin 150090，Heilongjiang；

3.Development Academy，Jilin Agricultural University，Changchun 130600，Jilin；

4.National Research Center of Soybean Engineering and Technology，Harbin 150050，Heilongjiang，China

Keywords:

Soybean; Aspartic acid; Key enzyme genes; Electronic mapping; Gene structure

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2010.01.0022

Abstract:

The nutritive value of soybean mainly focused on the aspartate-derived amino acids，and the content of these amino acids were affected by the key enzymes gene from aspartic acid metabolic pathway.In this research，the integrated map between soybean public genetic maps and physical maps was used，and the key enzyme gene sequences were blasted with soybean genome database by local Blast software.Seventeen key enzyme genes in aspartic acid metabolic pathway of soybean were mapped on soybean genetic map，and gene structures were analyzed firstly.The result indicated that these enzyme genes were mapped on 11 linkages group，including A2，B2，C2，D1b，D2，G，I，L，and M，respectively，and the flanking markers of these genes on the linkage group were obtained.The number of introns was from 0 to 13.There was no intron in AHAS，DHDPS，HK，and TS，otherwise there were 13 introns in DHAD，and 12 introns in AK.The corresponding markers obtained from mapping were the base of molecular assisted selection，while the structure information can make gene function analysis easily.

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