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Cloning and Analysis of PAL2 Gene from Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年01期
Page:
13-17
Research Field:
Publishing date:

Info

Title:
Cloning and Analysis of PAL2 Gene from Soybean
Author(s):
WANG Chan-chan; WANG An-na; WU Lei; LI Ye-cheng; LIU Cheng; MA Feng-ming
College of Agriculture，Northeast Agricultural University，Harbin 150030，Heilongjiang，China
Keywords:
Soybean Phenylalanine Ammonia-lyase(PAL); Sequence analysis
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.01.0013
Abstract:
The PAL2 gene，cloned from soybean by RT-PCR，is a new member of PAL gene family.It is named GmPAL2 and the accession number is GQ220305.The protein encoded by PAL2 was analyzed by informatics tools provided on internet.The complete cDNA of PAL2 was 2284bp and it encoded a polypeptide chain which contained 717 amino acid residues.The molecular weight of the protein encoded by PAL2 gene was 78.116kD.The homology of soybean PAL2 and bean PAL2 reached 92% by BLASTp tool，the homology of soybean PAL2 and soybean PAL1 was only 89%.The olotained PAL2 gene will be a base for the recombinant expression and the analysis of PAL gene family in soybean.

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Last Update: 2014-09-12