|Table of Contents|

Cloning and Transformation Study of Transcription Factor GmLEC1 in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2010年01期
Page:
7-12
Research Field:
Publishing date:

Info

Title:
Cloning and Transformation Study of Transcription Factor GmLEC1 in Soybean
Author(s):
ZHU Hong-lin1SHA Ai-hua2FU Xiu-mei1CHEN Yin-hua1SHAN Zhi-hui2ZHOU Xin-an2
1.Key Laboratory of Tropical Biological Resources,Ministry of Education,Hainan University,Haikou 570228 Hainan;
2.Institute of Oil Crops Research,Chinese Academy of Agriculture Sciences,Wuhan 430062,Hubei,China
Keywords:
SoybeanLEC1geneRNA interference vectorTransformationCotyledon nodeEmbryonic tips
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.01.0007
Abstract:
The developing seeds of high-oil soybean“Zhongdou 30” were employed in this study.Degenerate primers were designed according to the reported sequence of transcription factor LEC1 related to fatty acid synthesis in Arabidopsis.A 850 bp cDNA fragment was isolated from soybean seed by homology-based candidate gene method.And RNAi vector pHGlec was constructed by Gateway technology.And then the vector was delivered into soybean via Agrobacterium-mediated transformation,using cotyledonary nodes and embryonic tips of soybean as explants.The rate of resistant shoots and the positive rate of PCR was used as criteria for selection the proper conditions.The results indicated that the optimal system of shoots elongation was 6.0 mg?L-1 of 6-BA for embryonic tips.The positive rate of PCR by optimized embryonic tips transformation system was 7.58%,and it was 1.86% by cotyledon nodes transformation system.

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Last Update: 2014-09-12