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Analyse of Isoflavones Content in Normal Soybean Varieties（Lines）in Jilin Province(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年06期
Page:
1076-1080
Research Field:
Publishing date:

Info

Title:
Analyse of Isoflavones Content in Normal Soybean Varieties（Lines）in Jilin Province
Author(s):
SONG Zhi-feng1; WANG Li1; MENG Fan-gang2; WANG Xin-feng2; HUANG Huang1; MA Wei2; FU Jian2
1.Research Center of Agricultural Enviroment and Resource，Jilin Academy of Agricultural Sciences，Changchun 130033，Jilin；
2.Research Center of Soybean，Jilin Academy of Agricultural Sciences，Changchun 130033，Jilin，China
Keywords:
HPLC;  Soybean;  Isoflavone;  Determination
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.06.1076
Abstract:
A HPLC method for rapid determination of isoflavones from soybean is presented.The isoflavones were extracted by 80% methanol and followed hydrolyzing at 100℃ to complete conversion of the acetyl and malonyl forms of isoflavones to β-glucoside and aglucone forms.The isoflavones were separated on a C18 short column using gradient elution；column oven temperature，40℃；the diode array detector monitoring wavelength ，260 nm.The peak area of isoflavones versus their concentration had a good linearity（correlation coefficient were 0.9998-0.9999）in the range of 50-1000 μmol.L-1.Limits of detection(LOD）were in the range of 1.09-2.06 mg.L-1，limits of quantification(LOQ）were in the range of 3.25-6.46 mg.L-1.The recoveries of standard spiking of isoflavones were in the range of 97.11%-103.31%.The relative standard deviation(RSD，n=7）were in the range of 2.03%-4.32%.The linearity of the method was good in a wide range.This method has high analytic sensitivity and accuracy,and was suitable for determination of isoflavones in soybean and other cereal products.The isoflavone content in 261 Jilin province normal soybean varieties（lines）ranged from 1.46 mg.g-1.to 4.97 mg.g-1.The isoflavone content of 23 varieties（lines）soybean exceeded 4 mg.g-1.The research results indicated that the content range and characteristics of the soybean oligosaccharides in Jilin province and lay a foundation for the further research of soybean quality breeding and processing and utilization of soybean isoflavone.

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Last Update: 2014-09-12