|Table of Contents|

Response of PAL Activity to Phytophthora sojae Inoculation in Glycine soja(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年06期
Page:
1044-1048
Research Field:
Publishing date:

Info

Title:
Response of PAL Activity to Phytophthora sojae Inoculation in Glycine soja
Author(s):
ZHANG Shu-zhen123JIN Li-mei3XU Peng-fei3CHEN Wei-yuan4WU Jun-jiang5LI Wen-bin3QIU Li-juan6CHANG Ru-zhen6
3.Soybean Research Institute,Northeast Agricultural University,Key Laboratory of National Education Department,Harbin 150030;
4.Suihua Branch of Heilongjiang Academy of Agricultural Sciences,Suihua 152052;
5.Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086;
6.Crop Science Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China
Keywords:
Phytophthora root and stem rotGlycine sojaPALActivity change
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.06.1044
Abstract:
Phytophthora root and stem rot caused by Phytophthora sojae is a destructive disease in soybean production regions all around the world.Some protective enzymes begin to be active when soybean is infected by P.sojae.Of the enzymes,phenylalanine ammonia-lyase(PAL)is a key and rate-limiting enzyme in phenyl propane metabolize pathway which could produce kinds of antimicrobial products.There is abundant wild soybean in China,but there are few reports on the relationship about PAL and resistance of wild soybean to P.sojae.In this paper,4 resistant wild soybeans and 4 susceptible wild soybeans were inoculated with zoospores of P.sojae race 1 as treatment and those inoculated with water as control.The PAL activity in roots,stems,and leaves were measured after inoculation of 0,12,24,36,48,60,and 72 h in the treatment and control respectively.The results indicated that the PAL activity in roots and stems of resistant wild soybeans was higher than that of the control at most of the stages of pathogenetic process,but was lower than that of control for susceptible wild soybean.The change range of PAL activity in leaves of both susceptible and resistant wild soybeans were relatively low compared with that of control.

References:

[1]Schmitthenner A F.Problems and progress in control of phytophthora root rot of soybean[J].Plant Disease,1985,69:362-368.

[2]Hildebrand A A.A root and stalk rot caused by P.me.var.sojae[J].Canadian Journal of Botany,1959,37:927-957.
[3]Jee Hyeongjin.Occurance of Phytophthora root rot on soybean and indentification of the causal fungus[J].RAD Journal of Crop Protection,1998,40:16-22.
[4]Pegg K G,Kochman J K,Vock N T.Root and stem rot of soybean caused by Phytophthora megasperma var.sojae in Australia[J].Plant Pathology,1980,9:15.
[5]沈崇尧,苏彦纯.中国大豆疫霉病菌的发现及初步研究[J].植物病理学报,1991,21(3):298.(Shen C Y,Su Y C.Discovery and preliminaty studies of Phytophthora megasperma on soybean in China[J].Acta Phytopathologica Sinica,1991,21(3):298.)
[6]徐永华,卢启,何志鸿,等.大豆疫霉病早熟抗源[J].大豆通报,1999(3):23-25.(Xu Y H,Lu Q,He Z H,et al.Early-maturity soybean varieties resistance to Phytophthora sojae[J].Soybean Bulletin,1999(3):23-25.)
[7]李宝英,马淑梅,丁俊杰.大豆疫霉病发生危害及影响其发生因素的探讨[J].植物保护,1999,25(5):8-11.(Li B Y,Ma S M,Ding J J.Investigations of soybean phytophthora root rot disease and its determinant factors[J].Plant Protection,1999,25(5):8-11.)
[8]王敬文,薛应龙.植物苯丙氨酸解氨酶的研究Ⅰ[J].植物生理学报,1981,7(4):373-375.(Wang J W,Xue Y L.Studies on plant phenylalanine ammonia-lyase(PAL)-Ⅰ[J].Acta Photophysiologica Sinica,1981,7(4):373-375.)
[9]王敬文,薛应龙.植物苯丙氨酸解氨酶的研究Ⅱ[J].植物生理学报,1982,8(1):35-37.(Wang J W,Xue Y L.Studies on plant phenylalanine ammonia-lyase(PAL)- Ⅱ[J].Acta Photophysiologica Sinica,1982,8(1):35-37.)
[10]王敬文,薛应龙.植物苯丙氨酸解氨酶的研究Ⅲ[J].植物生理学报,1982,8(3):273-275.(Wang J W,Xue Y L.Studies on plant phenylalanine ammonia-lyase(PAL)- Ⅲ[J].Acta Photophysiologica Sinica,1982,8(3):273-275.)
[11]庄炳昌.中国野生大豆研究二十年[J].吉林农业科学,1999,24(5):3-10.(Zhuang B C.Researches on wild soybean( Glycine soja)in China for twenty years[J].Jilin Agricultural Sciences,1999,24(5):3-10.)
[12]Yang X B,Ruff R L,Meng X Q,et al.Races of Phytophthora sojae in Iowa soybean fields[J].Plant Disease,1996,80:1418-1420.
[13]左豫虎,臧忠婧,刘锡若.影响大豆疫霉菌游动孢子产生的条件[J].植物病理学报,2001,31(3):241-245.(Zuo Y H,Zang Z J,Liu T R.Studies on production condition of zoospores of Phytophthora sojae[J].Acta Phytopathologica Sinica,2001,31(3):241-245.)
[14]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.(Li H S.Principle and experimental technology of plant biochemistry[M].Beijing:Higher Education Press,2000.)
[15]郝再彬.植物生理学实验[M].哈尔滨:哈尔滨工业大学出版社,2004,24-38.(Hao Z B.Plant physiology experiment[M].Harbin:Harbin Institute of Technology Press,2004:24-38.)
[16]吴岳轩,曾宝华,王容臣.杂交稻对白叶枯病菌的诱导抗性与细胞内防御酶关系的初步研究[J].植物病理学报,1996,26(2):127-131.(Wu Y X,Zeng B H,Wang R C.A Preliminary study on the relationship between induced resistance to bacterial blight and defense enzymes in hybrid rice seedlings[J].Acta Phytopathologica Sinica,1996,26(2):127-131.)
[17]贺字典,高增贵,庄敬华,等.玉米丝黑穗病菌对寄主防御相关酶活性的影响[J].玉米科学,2006,14(2): 150-155.(He Z D,Gao Z G,Zhuang J H,et al.Effect of maize head smut pathology(Sphacelotheca reiliana)on the major defensive enzymes of host[J].Journal of Maize Sciences,2006,14(2):150-155.)
[18]曾永三,王振中.豇豆与锈菌互作中的多酚氧化酶和过氧化物酶活性及其与抗病性的关系[J].植物保护学报,2004,31(2):145-150.(Zeng Y S,Wang Z Z.Relationships between activities of polyphenol oxidase and peroxidase,and resistance of cowpea to Uromyces vignae[J].Journal of Plant Protection,2004,31(2):145-150.)
[19]崔彦玲,张环.番茄叶霉病抗性与苯丙氨酸解氨酶的相关性[J].华北农学报,2003,18(1):79-82.(Cui Y L,Zhang H.Correlation analyses between resistance to cladosporium fulvum and PAL activity in tomato[J].Acta Agriculturae Boreall-Sinica,2003,18(1):79-82.)

Memo

Memo:
-
Last Update: 2014-09-12