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Expression of CP4 EPSPS Protein of Genetically Modified Roundup Ready Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年06期
Page:
981-984,989
Research Field:
Publishing date:

Info

Title:
Expression of CP4 EPSPS Protein of Genetically Modified Roundup Ready Soybean
Author(s):
XING Zhen-juanLI Fei- wuLIU NaLI Cong-congKANG Ling-shengSONG Xin-yuanSHAO Gai-geZHANG Ming
Jilin Academy of Agricultural Sciences,National Center for Environmental Safety Inspection of Transgenic Plants,Ministry of Agriculture,Changchun 130033,Jilin,China
Keywords:
Transgenic soybeanCP4 EPSPS protein contentEnzyme-linked immunosorbent assay
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.06.981
Abstract:
The content of CP4 EPSPS protein in transgenic soybean plant was studied by Elisa method in this paper.Results showed that significant difference of CP4 EPSPS protein contents existed in different tissues and growth stages,with CP4 EPSPS protein content in seeds at maturity the highest.The contents of CP4 EPSPS protein in the upper and the bottom leaves were not significantly different in all growth stages except stages V3-V5 and R8.The contents of CP4 EPSPS protein in the upper and the bottom stalks showed consistent trend in different stages.The content of CP4 EPSPS protein in root decreased in stage V3-V5,then increased gradually,and then decreased from R1 to R8.With the growth of the plant,the content of the protein of CP4 EPSPS in all tissues changed greatly from V1 to V8 stage.

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Last Update: 2014-09-11